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APC Mouse Anti-Human CD133
APC Mouse Anti-Human CD133
CD133 expression on human WERI-Rb-1cells      Panel 1. Cells from the WERI-Rb-1 (Retinoblastoma, ATCC HTB-169) cell line were stained with either APC Mouse IgG1, κ Isotype Control (Cat. No. 554681; dashed line histogram) or APC Mouse Anti-Human CD133 antibody (Cat. No. 566596/566597; solid line histogram) at 0.06 μg/test. The fluorescence histogram showing CD133 expression (or Ig Isotype control staining) was derived from gated events with forward and side light-scatter characteristics of viable cells. CD133 expression on human peripheral blood mononuclear cells      Panel 2A. Human peripheral blood mononuclear cells (PBMC) were stained with PE Mouse Anti-Human CD34 antibody (Cat. No. 348057) and either APC Mouse IgG1, κ Isotype Control (Left Plot) or APC Mouse Anti-Human CD133 (Right Plot) at 0.06 μg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells before analysis. Pseudocolor dot plots showing the correlated expression of CD133 (or Ig Isotype control staining) versus CD34 were derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD negative) peripheral blood lymphocytes (PBL).      Panel 2B. Human PBMC were similarly stained with BD Horizon™ BUV395 Mouse Anti-Human CD45 antibody (Cat. No. 563791/563792) and either APC Mouse IgG1, κ Isotype Control (Left Plot) or APC Mouse Anti-Human CD133 antibody (Right Plot) at 0.06 μg/test followed by Cell Viability 7-AAD Solution addition before analysis. Pseudocolor dot plots showing the correlated expression of CD45 versus CD133 (or Ig Isotype control staining) were similarly derived for viable PBL.      Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
CD133 expression on human WERI-Rb-1cells      Panel 1. Cells from the WERI-Rb-1 (Retinoblastoma, ATCC HTB-169) cell line were stained with either APC Mouse IgG1, κ Isotype Control (Cat. No. 554681; dashed line histogram) or APC Mouse Anti-Human CD133 antibody (Cat. No. 566596/566597; solid line histogram) at 0.06 μg/test. The fluorescence histogram showing CD133 expression (or Ig Isotype control staining) was derived from gated events with forward and side light-scatter characteristics of viable cells. CD133 expression on human peripheral blood mononuclear cells      Panel 2A. Human peripheral blood mononuclear cells (PBMC) were stained with PE Mouse Anti-Human CD34 antibody (Cat. No. 348057) and either APC Mouse IgG1, κ Isotype Control (Left Plot) or APC Mouse Anti-Human CD133 (Right Plot) at 0.06 μg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells before analysis. Pseudocolor dot plots showing the correlated expression of CD133 (or Ig Isotype control staining) versus CD34 were derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD negative) peripheral blood lymphocytes (PBL).      Panel 2B. Human PBMC were similarly stained with BD Horizon™ BUV395 Mouse Anti-Human CD45 antibody (Cat. No. 563791/563792) and either APC Mouse IgG1, κ Isotype Control (Left Plot) or APC Mouse Anti-Human CD133 antibody (Right Plot) at 0.06 μg/test followed by Cell Viability 7-AAD Solution addition before analysis. Pseudocolor dot plots showing the correlated expression of CD45 versus CD133 (or Ig Isotype control staining) were similarly derived for viable PBL.      Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Product Details
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BD Pharmingen™
PROM1; PROML1; prominin-1; AC133; CORD12; MCDR2; MSTP061; RP41; STGD4
Human (QC Testing)
Mouse BALB/c IgG1, κ
WERI-RB-1 Retinoblastoma Cell
Flow cytometry (Routinely Tested)
0.2 mg/ml
VII 70485
8842
AB_2744280
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Antibody Details
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W6B3C1

The W6B3C1 monoclonal antibody specifically recognizes CD133 which is also known as Prominin-like protein 1 (PROML1), Prominin-1 (PROM1), hProminin, Hematopoietic stem cell antigen, Macular dystrophy retinal 2 (MCDR2), Stargardt disease 4 autosomal dominant (STGD4), or AC133 antigen. CD133 is an ~120 kDa five-transmembrane, glycoprotein that is encoded by PROM1 (Prominin 1) which belongs to the Prominin gene family. This single-chain, pentaspan transmembrane glycoprotein is comprised of an extracellular N-terminus with two short intracellular sequences and two long extracellular loops followed by an intracellular C-terminus. CD133 is expressed on some cells found in a variety of tissues including the bone marrow, cord and peripheral blood, placenta, liver, pancreas, kidney, lung, retina, brain and heart. It is expressed on various cell types including hematopoietic stem cells and progenitor cells, neural stem cells, developing epithelial cells, precursor endothelial cells, and retinal cells. CD133 is expressed on some cancer cells found in leukemias, melanoma and retinoblastoma. It may serve as a cancer stem cell marker in a number of brain tumors, melanoma, colon cancer, hepatocellular carcinoma, pancreatic adenocarcinoma, and prostate cancer. A mutation in PROM1 is reportedly associated with a form of human retinal degeneration. The W6B3C1 antibody recognizes a different epitope than the human CD133-specific 293C3 antibody.

        

Format Details
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APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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APC
Red 627-640 nm
651 nm
660 nm
Citations & References
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Development References (5)

  1. Bühring HK, Marzer A, Lammers R, Wissinger B. CD133 cluster report. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:622-623.
  2. Koerner SP, André MC, Leibold JS, et al. An Fc-optimized CD133 antibody for induction of NK cell reactivity against myeloid leukemia.. Leukemia. 2017; 31(2):459-469. (Clone-specific: Blocking, Flow cytometry). View Reference
  3. Lammers R, Giesert C, Grünebach F, Marxer A, Vogel W, Bühring HJ. Monoclonal antibody 9C4 recognizes epithelial cellular adhesion molecule, a cell surface antigen expressed in early steps of erythropoiesis.. Exp Hematol. 2002; 30(6):537-45. (Immunogen: Flow cytometry). View Reference
  4. Maw MA, Corbeil D, Koch J, et al. A frameshift mutation in prominin (mouse)-like 1 causes human retinal degeneration.. Hum Mol Genet. 2000; 9(1):27-34. (Biology). View Reference
  5. Miraglia SJ, Buck D. CD133 (AC133). In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:870-872.
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.