Alexa Fluor® 700 Mouse Anti-Human IgD
Clone IA6-2 (also known as δ-IA6-2)(RUO)
Flow cytometric analysis of IgD expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were incubated in complete tissue culture medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with Alexa Fluor® 488 Mouse anti-Human CD19 antibody (Cat. No. 557697) and with either an Alexa Fluor® 700 Mouse IgG2a, κ Isotype Control (Cat. No. 557880; Left Panel) or an Alexa Fluor® 700 Mouse anti-Human IgD antibody (Cat. No. 561302; Right Panel). The two-color flow cytometric dot plots showing the correlated expression of IgD (or Ig isotype control staining) versus CD19 were derived from events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
BD Pharmingen™ Alexa Fluor® 700 Mouse Anti-Human IgD
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.