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Alexa Fluor® 647 Rat anti-Mouse MD-1
Alexa Fluor® 647 Rat anti-Mouse MD-1
Flow cytometric analysis of Alexa Fluor® 647-conjugated anti-Mouse MD-1 on mouse lymph node cells.  Mouse lymph nodes were stained with FITC-conjugated anti-mouse CD22.2 (Clone Cy34.1, Cat No. 557474) and Alexa Fluor® 647-conjugated anti-mouse MD-1 antibody (Clone MD14, Cat. No. 558204; right panel) or Alexa Fluor® -conjugated rat IgG2a (Clone R35-95, Cat. No. 557690; left panel). Flow cytometry was performed  on a BD™ FACSCalibur flow cytometry system and the plots were derived from the gated events based on light scattering characteristics of viable splenocytes.
Flow cytometric analysis of Alexa Fluor® 647-conjugated anti-Mouse MD-1 on mouse lymph node cells.  Mouse lymph nodes were stained with FITC-conjugated anti-mouse CD22.2 (Clone Cy34.1, Cat No. 557474) and Alexa Fluor® 647-conjugated anti-mouse MD-1 antibody (Clone MD14, Cat. No. 558204; right panel) or Alexa Fluor® -conjugated rat IgG2a (Clone R35-95, Cat. No. 557690; left panel). Flow cytometry was performed  on a BD™ FACSCalibur flow cytometry system and the plots were derived from the gated events based on light scattering characteristics of viable splenocytes.
Product Details
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BD Pharmingen™
Ly-86
Mouse (QC Testing)
Rat IgG2a, κ
Normal Rat Kidney (NKR)
Flow cytometry (Routinely Tested)
0.2 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  3. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
558204 Rev. 1
Antibody Details
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MD14

The monoclonal antibody MD14 reacts with MD-1, a secretory molecule that associates with RP105 (CD180) on the surface of B cells.  RP105 belongs to the LRR superfamily, in which some members are implicated in linking innate and adaptive immunity. The coexpression of MD-1 is essential for cell surface expression of RP105 and also for LPS recognition and signaling. The MD14 antibody has been found to be antagonistic in LPS-induced proliferation and B7.2 (CD86) up-regulation further suggesting that MD-1 helps RP105 to signal LPS.

558204 Rev. 1
Format Details
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Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
558204 Rev.1
Citations & References
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Development References (3)

  1. Miyake K, Shimazu R, Kondo J, et al. Mouse MD-1, a molecule that is physically associated with RP105 and positively regulates its expression.. J Immunol. 1998; 161(3):1348-1353. (Biology). View Reference
  2. Miyake K, Yamashita Y, Ogata M, Sudo T, Kimoto M. RP105, a novel B cell surface molecule implicated in B cell activation, is a member of the leucine-rich repeat protein family. J Immunol. 1995; 154(7):3333-3340. (Biology). View Reference
  3. Nagai Y, Shimazu R, Ogata H et al. Requirement for MD-1 in cell surface expression of RP105/CD180 and B-cell responsiveness to lipopolysaccharide. Blood. 2002; 99(5):1699-1705. (Biology). View Reference
558204 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.