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Alexa Fluor® 647 Rat Anti-Mouse GITR Ligand
Alexa Fluor® 647 Rat Anti-Mouse GITR Ligand

Flow cytometric analysis of mouse GITR Ligand expression on non-transfected and GITR Ligand-transfected cells. Non-transfected (Left Panel) and GITR Ligand-transfected (Right Panel) P815 cells were stained with either Alexa Fluor® 647 Rat IgG2b, κ Isotype Control (Cat. No. 557691) or Alexa Fluor® 647 Rat Anti-Mouse GITR Ligand antibody (Cat. No. 563542). Fluorescence histograms showing the expression of GITR Ligand (solid line histograms) or Ig Isotype control staining (dashed line histograms) on non-transfected or GITR Ligand-transfected P815 cells were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analyses were performed using a BD™ LSR II Flow Cytometer System.

Flow cytometric analysis of mouse GITR Ligand expression on non-transfected and GITR Ligand-transfected cells. Non-transfected (Left Panel) and GITR Ligand-transfected (Right Panel) P815 cells were stained with either Alexa Fluor® 647 Rat IgG2b, κ Isotype Control (Cat. No. 557691) or Alexa Fluor® 647 Rat Anti-Mouse GITR Ligand antibody (Cat. No. 563542). Fluorescence histograms showing the expression of GITR Ligand (solid line histograms) or Ig Isotype control staining (dashed line histograms) on non-transfected or GITR Ligand-transfected P815 cells were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analyses were performed using a BD™ LSR II Flow Cytometer System.

Product Details
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BD Pharmingen™
Tnfsf18; Gitrl Glucocorticoid-induced-tumor necrosis factor receptor ligand
Mouse (QC Testing)
Rat SD, also known as Sprague-Dawley (outbred) IgG2b, κ
Mouse GITRL Transfected Cell Line
Flow cytometry (Routinely Tested)
0.2 mg/ml
240873
AB_2738270
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Product Notices

  1. An isotype control should be used at the same concentration as the antibody of interest.
  2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  5. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  6. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
  7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
563542 Rev. 1
Antibody Details
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MIH44

The MIH44 monoclonal antibody specifically binds to Glucocorticoid-induced TNF-related Ligand (GITR Ligand). The GITR Ligand protein is encoded by the Tnfsf18 (Tumor necrosis factor ligand superfamily member 18) gene. The GITR Ligand is a type II transmembrane protein that is expressed on endothelial cells and antigen presenting cells, including B cells, macrophage and dendritic cells. The GITR Ligand reportedly costimulates T cell activation and reverses CD4+CD25+ Treg cell-mediated suppression by interaction with its cognate receptor, GITR that is expressed by these cells.

563542 Rev. 1
Format Details
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Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
563542 Rev.1
Citations & References
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Development References (2)

  1. Igarashi H, Cao Y, Iwai H, et al. GITR ligand-costimulation activates effector and regulatory functions of CD4+ T cells. Biochem Biophys Res Commun. 2008; 369(4):1134-1138. (Immunogen: Blocking, Flow cytometry, Inhibition). View Reference
  2. Piao J, Kamimura Y, Iwai H, et al. Enhancement of T-cell-mediated anti-tumour immunity via the ectopically expressed glucocorticoid-induced tumour necrosis factor receptor-related receptor ligand (GITRL) on tumours. Immunology. 2009; 127(4):489-499. (Clone-specific: Flow cytometry). View Reference
563542 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.