Two-color flow cytometric analysis of CD206 expression on mouse peritoneal exudate cells. Thioglycollate-elicited BALB/c mouse peritoneal exudate cells (PEC) were fixed and permeabilized using the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725). The leucocytes were then stained with PE Rat Anti-Mouse CD107b (Mac-3) antibody (Cat. No. 553324) and either Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (Cat. No. 565250; Left Panel) or Alexa Fluor® 647 Rat Anti-Mouse CD206 antibody (Cat. No. 565250; Right Panel). Two-color flow cytometric contour plots showing the correlated expression of CD206 (or Ig Isotype control staining) versus CD107b were derived from gated events with the forward and side-light scattering characteristics of intact PEC. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
Flow cytometric analysis of CD206 expression by J774A cells. Cells from the J774A (Mouse macrophage, ATCC TIB-67) cell line were similarly fixed and permeabilized, stained with either Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (dashed line histogram) or Alexa Fluor® 647 Rat Anti-Mouse CD206 antibody (solid line histogram), and analzyed by flow cytometry. The fluorescence histogram showing CD206 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact J774A cells.
Two-color flow cytometric analysis of CD206 expression on mouse peritoneal exudate cells. Thioglycollate-elicited BALB/c mouse peritoneal exudate cells (PEC) were fixed and permeabilized using the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725). The leucocytes were then stained with PE Rat Anti-Mouse CD107b (Mac-3) antibody (Cat. No. 553324) and either Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (Cat. No. 565250; Left Panel) or Alexa Fluor® 647 Rat Anti-Mouse CD206 antibody (Cat. No. 565250; Right Panel). Two-color flow cytometric contour plots showing the correlated expression of CD206 (or Ig Isotype control staining) versus CD107b were derived from gated events with the forward and side-light scattering characteristics of intact PEC. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
Flow cytometric analysis of CD206 expression by J774A cells. Cells from the J774A (Mouse macrophage, ATCC TIB-67) cell line were similarly fixed and permeabilized, stained with either Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (dashed line histogram) or Alexa Fluor® 647 Rat Anti-Mouse CD206 antibody (solid line histogram), and analzyed by flow cytometry. The fluorescence histogram showing CD206 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact J774A cells.
Product Details
BD Pharmingen™
MMR; MR; Macrophage mannose receptor 1; Mrc1; Mannose receptor, C type 1
Mouse (QC Testing)
Rat F344, also known as Fischer, CDF IgG2a
Recombinant Mouse CD206 Carbohydrate recognition domains 4-7/Fc Fusion Protein
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
AB_2739133
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Transcription Factor Buffer Set RUO
Size
100 Tests
Cat No.
562574
Alexa Fluor® 647 Rat IgG2a, κ Isotype Control RUO
Size
0.1 mg
Cat No.
557690
Transcription Factor Buffer Set RUO
Size
25 Tests
Cat No.
562725
PE Rat Anti-Mouse CD107b RUO
Size
0.2 mg
Cat No.
553324
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565250 Rev. 1
Antibody Details
MR5D3
The MR5D3 monoclonal antibody specifically binds to CD206 which is also known as the Macrophage mannose receptor (MMR, MR) or Mannose receptor, C type 1 (Mrc1). CD206 is a type I transmembrane glycoprotein of approximately 175 kDa that belongs to the C-type lectin superfamily. It is expressed at the cell surface and intracellularly by macrophages, Langerhans cells, dendritic cells, and endothelial cells within hepatic and lymphoid tissues. This pattern recognition receptor binds to endogenous and microbial glycoconjugates containing mannose, fucose, or N-acetylglucosamine through its C-type lectin-like carbohydrate recognition domains (CRD). CD206 also contains a cysteine-rich domain that enables binding to sulfated carbohydrate antigens. This receptor enables macrophages and other specialized cells to maintain tissue homeostasis as well as to internalize microbes or their components by phagocytosis or endocytosis. CD206 thus plays important roles in mediating innate immunity, e.g., enabling phagocytosis, as well as in processing and presenting antigens for the generation and expression of adaptive immunity. Moreover, CD206 has been associated with leucocyte homing and cancer cell metastasis.
565250 Rev. 1
Format Details
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
565250 Rev.1
Citations & References
View product citations for antibody "565250" on CiteAb
Development References (5)
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Akbarshahi H, Menzel M, Posaric Bauden M, Rosendahl A, Andersson R. Enrichment of murine CD68+ CCR2+ and CD68+ CD206+ lung macrophages in acute pancreatitis-associated acute lung injury. PLoS ONE. 2012; 7(10):e42654. (Biology). View Reference
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Burgdorf S, Lukacs-Kornek V, Kurts C. The mannose receptor mediates uptake of soluble but not of cell-associated antigen for cross-presentation. J Immunol. 2006; 176(11):6770-6776. (Biology). View Reference
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Marttila-Ichihara F, Turja R, Miiluniemi M, et al. Macrophage mannose receptor on lymphatics controls cell trafficking. Blood. 2008; 112(1):64-72. (Clone-specific: Immunofluorescence, Immunohistochemistry). View Reference
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McKenzie EJ, Taylor PR, Stillion RJ, et al. J Immunol. 2007; 178(8):4975-4983. (Clone-specific: Flow cytometry). View Reference
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Zamze S, Martinez-Pomares L, Jones H, et al. Recognition of bacterial capsular polysaccharides and lipopolysaccharides by the macrophage mannose receptor. J Biol Chem. 2002; 277(44):41613-41623. (Immunogen: Dot Blot, ELISA, Flow cytometry, Immunoaffinity chromatography, Immunohistochemistry, Immunoprecipitation). View Reference
565250 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.