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Alexa Fluor® 488 Mouse anti-PDX-1
Alexa Fluor® 488 Mouse anti-PDX-1

Flow cytometric analysis of PDX-1 in mouse pancreatic tumor (insulinoma) cells. Beta-TC-6 (ATCC CRL-11506) cells were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm buffer III (Cat. No. 558050). The cells were stained with either Alexa Fluor® 488 Mouse IgG1,  isotype control (Cat. No. 557721, dashed line) or Alexa Fluor® 488 Mouse anti-PDX-1 monoclonal antibody (Cat.. No. 562274, solid line) at matched concentrations. Flow cytometry was performed on a BD LSRFortessa™ flow cytometry system. This antibody has been found to be reactive with both mouse and human PDX-1, and it can also be used on fixed cells that were permeabilized with BD Phosflow™ Perm/Wash Buffer I (Cat. No. 557885).

Flow cytometric analysis of PDX-1 in mouse pancreatic tumor (insulinoma) cells. Beta-TC-6 (ATCC CRL-11506) cells were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm buffer III (Cat. No. 558050). The cells were stained with either Alexa Fluor® 488 Mouse IgG1,  isotype control (Cat. No. 557721, dashed line) or Alexa Fluor® 488 Mouse anti-PDX-1 monoclonal antibody (Cat.. No. 562274, solid line) at matched concentrations. Flow cytometry was performed on a BD LSRFortessa™ flow cytometry system. This antibody has been found to be reactive with both mouse and human PDX-1, and it can also be used on fixed cells that were permeabilized with BD Phosflow™ Perm/Wash Buffer I (Cat. No. 557885).

Product Details
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BD Pharmingen™
PDX1, GSF, IPF-1, IPF1, IUF-1, IDX-1, STF-1
Mouse (QC Testing), Human (Tested in Development)
Mouse IgG1, κ
Human PDX-1 Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested), Bioimaging, Immunofluorescence (Not Recommended)
5 µl
3651
AB_10924596
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  6. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  7. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  8. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
562274 Rev. 2
Antibody Details
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658A5

The 658A5 monoclonal antibody binds PDX-1 or Pancreas/Duodenum Homeobox Protein-1 encoded by the PDX1 or IPF1 (Insulin Promoter Factor 1) gene. PDX-1 is a key regulator of pancreatic development and adult beta-cell function. Loss of PDX1 gene function in mice and humans results in pancreatic agenesis. PDX-1 is also involved in endocrine precursor cell development by binding the DNA-binding transcription factor Neurogenin-3. PDX-1-positive cells have been obtained by the directed differentiation of human embryonic stem cells through a definitive endoderm lineage.

562274 Rev. 2
Format Details
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Alexa Fluor™ 488
Alexa Fluor™ 488 Dye is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 517-nm. Alexa Fluor™ 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520-nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 488
Blue 488 nm
494 nm
517 nm
562274 Rev.2
Citations & References
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Development References (5)

  1. Babu DA, Deering TG, Mirmira RG. A feat of metabolic proportions: Pdx1 orchestrates islet development and function in the maintenance of glucose homeostasis. Mol Genet Metab. 2007; 92(1-2):43-55. (Biology). View Reference
  2. D'Amour KA, Bang AG, Eliazer S, et al . Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells. Nat Biotechnol. 2006; 24(12):1481-1483. (Biology: Calcium Flux). View Reference
  3. Gannon M, Ables ET, Crawford L, et al. pdx-1 function is specifically required in embryonic beta cells to generate appropriate numbers of endocrine cell types and maintain glucose homeostasis. Dev Biol. 2008; 314(2):406-417. (Biology). View Reference
  4. Gu G, Dubauskaite J, Melton DA. Direct evidence for the pancreatic lineage: NGN3+ cells are islet progenitors and are distinct from duct progenitors. Development. 2002; 129(10):2447-2457. (Biology). View Reference
  5. Ohlsson H, Karlsson K, Edlund T. IPF1, a homeodomain-containing transactivator of the insulin gene. EMBO J. 1993; 12(11):4251-4259. (Biology). View Reference
View All (5) View Less
562274 Rev. 2

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.