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Ig Isotype Control Cocktail - A

Ig Isotype Control Cocktail - A

(RUO)
Product Details
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BD Pharmingen™
Flow cytometry (Tested During Development)
RUO
AB_1645629


Description

        Cocktail Component                Clone                

        APC mIgM                                G155-228                

        PE mIgG1                                MOPC-21        

        FITC mIgG1                                MOPC-21

The Isotype Control Cocktail A is a mixture of Mouse IgG1 and Mouse IgM designed and optimized to work in conjunction with the Rat T/B/NK Lymphocyte Cocktail (Cat. No. 558494).  The mouse IgM G155-228 is a monoclonal antibody specific for trinitrophenol (TNP), a hapten not expressed on mammalian cells. The mouse IgG1 MOPC-21 immunoglobulin is a mouse myeloma protein. All immunoglobulins were selected as isotype controls following screening for low background on a variety of mouse and human tissues.

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
558509 Rev. 2
Citations & References
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Development References (1)

  1. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology). View Reference
558509 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.