Skip to main content Skip to navigation
FITC Mouse Anti-Human NPM-ALK/ALK Set
Alert icon
This SKU will be discontinuing Jul 2022 or until supply ends. For additional support, contact your local applications specialist. Contact us #

FITC Mouse Anti-Human NPM-ALK/ALK Set

Product Details
Down Arrow Up Arrow

BD Pharmingen™
Human (QC Testing)
Intracellular staining (flow cytometry) (Routinely Tested)


Reacts with NPM-ALK, a chimeric gene product of approximately 80 kDa, which is the result of t(2;5)(p23;q35) chromosomal translocation. The gene at 5q35 encodes for the cell cycle-regulated nucleolar protein, nucleophosmin (NPM), while the expression of NPM-ALK protein, or p80, comprised of the N-terminal portion of NPM (about 40% of the molecule) linked to the entire intracellular portion of ALK. ALK1 antibody recognizes a formalin resistant epitope in both the NPM-ALK chimeric and the 200 kDa normal human ALK proteins. Cytoplasmic and nuclear expression of NPM-ALK is seen in t(2;5)+ cell lines Su-DHL-1 and Karpas 299.

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.

Recommended Assay Procedures

We recommend to use the Cytofix/cytoperm solution (Cat. No. 554714) for the cell fixation and permeabilization. Please refer to for technical protocols.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at
  5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  6. An isotype control should be used at the same concentration as the antibody of interest.
559818 Rev. 6
Down Arrow Up Arrow
Description Quantity/Size Part Number EntrezGene ID
FITC-Mouse Anti-Human NPM-ALK 100 Tests (1 ea) 51-37594X N/A
FITC Mouse IgG3 Kappa Isotype Control 100 Tests (1 ea) 51-35434X N/A
559818 Rev. 6
Citations & References
Down Arrow Up Arrow

Development References (3)

  1. Morris SW, Kirstein MN, Valentine MB, et al. Fusion of a kinase gene, ALK, to a nucleolar protein gene, NPM, in non-Hodgkin's lymphoma. Science. 1994; 263(5151):1281-1284. (Biology). View Reference
  2. Pittaluga S, Wlodarska I, Pulford K, et al. The monoclonal antibody ALK1 identifies a distinct morphological subtype of anaplastic large cell lymphoma associated with 2p23/ALK rearrangements. Am J Pathol. 1997; 151(2):343-351. (Biology). View Reference
  3. Pulford K, Lamant L, Morris SW, et al. Detection of anaplastic lymphoma kinase (ALK) and nucleolar protein nucleophosmin (NPM)-ALK proteins in normal and neoplastic cells with the monoclonal antibody ALK1. Blood. 1997; 89(4):1394-1404. (Biology). View Reference
559818 Rev. 6

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.