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Purified Mouse IgG3, κ Isotype Control
Product Details
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BD Pharmingen™
Mouse BALB/c IgG3, κ
ELISA Standard, Flow cytometry, Isotype control (Routinely Tested), Immunohistochemistry, Intracellular staining (flow cytometry) (Not Recommended)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

An isotype control should be used at the same concentration as the antibody of interest. This product is not recommended for use as an isotype control for intracellular or immunohistochemical staning applications. For immunohistochemical staining, we suggest the purified mouse IgG3, κ myeloma protein J606 in our special formulation for immunohistochemistry, Cat. No. 550341. We recommend Cat. No. 555577 for immunofluorescent staining of human whole blood and Cat. No. 556657 for non-human primate blood.

Caution: Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE™ (No Azide/Low Endotoxin) antibody format, (Cat. No. 553466) for in vitro and in vivo use.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
553486 Rev. 7
Antibody Details
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The A112-3 clone is specific for keyhole limpet hemocyanin, (KLH).  TNP-KLH, the immunogen, is not expressed on human, mouse, or rat cells.  In the absence of specific binding, this antibody may bind non-specifically to Fc receptors. The immunoglobulin from clone A112-3 was selected as an isotype control following screening for low background on a variety of mouse and human tissues.

This antibody is routinely tested by flow cytometric analysis and ELISA. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

553486 Rev. 7
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
553486 Rev.7
Citations & References
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553486 Rev. 7

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.