Flow cytometric analysis of BD Pharmigen™ FITC Mouse IgG3, ĸ Isotype Control on peripheral blood lymphocytes of rhesus macaque (Macaca mulatta). Peripheral blood lymphocytes were stained with BD Pharmigen FITC Mouse IgG3, ĸ Isotype control (Cat. No. 556658, solid line), or left unstained (dotted line). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphocytes.
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Flow cytometric analysis of BD Pharmigen™ FITC Mouse IgG3, ĸ Isotype Control on peripheral blood lymphocytes of rhesus macaque (Macaca mulatta). Peripheral blood lymphocytes were stained with BD Pharmigen FITC Mouse IgG3, ĸ Isotype control (Cat. No. 556658, solid line), or left unstained (dotted line). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphocytes.
Product Details
BD Pharmingen™
Anti-Fructosan
Rhesus, Cynomolgus, Baboon (QC Testing)
Mouse BALB/c IgG3, κ
Flow cytometry, Isotype control (Routinely Tested)
20 µl
AB_479692
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Lysing Solution 10X Concentrate IVD
Size
100 mL
Cat No.
349202
Lysing Buffer RUO
Size
100 mL
Cat No.
555899
556658 Rev. 6
Antibody Details
J606
Mouse IgG3 κ isotype control antibody is specific for the β-2 - 6-linked Fructosan. The transplantable plasmacytoma J606 was induced by intraperitoneal injection of mineral oils into BALB/c mice.
556658 Rev. 6
Format Details
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
556658 Rev.6
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
