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PerCP-Cy™5.5 Mouse Anti-Human CD28
Product Details
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BD™
CD28 antigen; T44; Tp44; TP44; Leu28
Human
Mouse BALB/c IgG1, κ
HPB-ALL T cell line
Flow cytometry
6 μg/mL
20 μL
7099,940
Phosphate buffered saline with gelatin and 0.1% sodium azide.
RUO (GMP)


Preparation And Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

337181 Rev. 1
Antibody Details
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L293

The CD28 antibody, clone L293, is derived from hybridization of Sp2/0-Ag14 mouse myeloma cells with spleen cells from BALB/c mice immunized with the HPB-ALL T-cell line.

The CD28 antigen, a disulfide-linked homodimeric glycoprotein, Mr 44 kilodaltons (kd), is a cell-adhesion molecule (CAM) and functions as the ligand for CD80 (B7-1) and CD86 (B7-2) antigens, which are present on activated B lymphocytes, monocytes, and dendritic cells. Interaction of the CD28 antigen with CD80 or CD86 antigens, or both, co-stimulates CD2 and CD3 antigen/T-cell antigen receptor (TCR)–dependent T-cell–mediated proliferation and cytotoxicity.

337181 Rev. 1
Format Details
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PerCP-Cy5.5
PerCP-Cy5.5 dye is part of the BD blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. PerCP-Cy5 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PerCP-Cy5.5
Blue 488 nm
482 nm
676 nm
337181 Rev.1
Citations & References
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View product citations for antibody "337181" on CiteAb

Development References (35)

  1. Appay V, Papagno L, Spina CA, et al. Dynamics of T cell responses in HIV infection. J Immunol. 2002; 168:3660-3666. (Biology).
  2. Azuma M, Cayabyab M, Buck D, Phillips JH, Lanier LL. CD28 interaction with B7 costimulates primary allogeneic proliferative responses and cytotoxicity mediated by small, resting T lymphocytes.. J Exp Med. 1992; 175(2):353-60. (Biology). View Reference
  3. Azuma M, Phillips JH, Lanier LL. CD28- T lymphocytes: antigenic and functional properties. J Immunol. 1993; 150:1147-1159. (Biology).
  4. Caux C, Vanbervliet B, Massacrier C, et al. B70/B7-2 is identical to CD86 and is the major functional ligand for CD28 expressed on human dendritic cells. J Exp Med. 1994; 180:1841-1847. (Biology).
  5. Centers for Disease Control. Perspectives in disease prevention and health promotion update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in health-care settings. MMWR. 1988; 37:377-388. (Biology).
  6. Clinical and Laboratory Standards Institute. 2005. (Biology).
  7. Filaci G, Fravega M, Negrini S, et al. Nonantigen specific CD8+ T suppressor lymphocytes originate from CD8+CD28– T cells and inhibit both T-cell proliferation and CTL function. Human Immunol. 2004; 65:142-156. (Biology).
  8. Fleischer J, Soeth E, Reiling N, Grage-Griebenow E, Flad HD, Ernst M. Differential expression and function of CD80 (B7-1) and CD86 (B7-2) on human peripheral blood monocytes. Immunology. 1996; 89:592-598. (Biology).
  9. Freeman GJ, Freedman AS, Segil JM, Lee G, Whitman JF, Nadler LM. B7, a new member of the Ig superfamily with unique expression on activated and neoplastic B cells. J Immunol. 1989; 143:2714-2722. (Biology).
  10. Gmünder H, Lesslauer W. A 45-kDa human T-cell membrane glycoprotein functions in the regulation of cell proliferative responses. Eur J Biochem. 1984; 142:153-160. (Biology).
  11. Hansen JA, Martin PJ, Nowinski RC. Monoclonal antibodies identifying a novel T-cell antigen and Ia antigens of human lymphocytes. Immunogenetics. 1980; 10:247-260. (Biology).
  12. Hara R, Fu SM, Hansen JA. Human T-cell activation, II: A new activation pathway used by a major T-cell population via a disulfide-bonded dimer of a 44-kilodalton polypeptide (9-3 antigen). J Exp Med. 1985; 161:1513-1524. (Biology).
  13. June CH, Ledbetter JA, Linsley PS, Thompson CB. Role of the CD28 receptor in T-cell activation. Immunol Today. 1990; 11:211-216. (Biology).
  14. Lanier LL, O'Fallon S, Somoza C, et al. CD80 (B7) and CD86 (B70) provide similar co-stimulatory signals for T cell proliferation, cytokine production, and generation of CTL. J Immunol. 1995; 154:97-105. (Biology).
  15. Levine BL, Ueda Y, Craighead N, Huang ML, June CH. CD28 ligands CD80 (B7-1) and CD86 (B7-2) induce long-term autocrine growth of CD4+ T cells and induce similar patterns of cytokine secretion in vitro. Int Immunol. 1995; 7:891-904. (Biology).
  16. Linsley PS, Brady W, Grosmaire L, Aruffo A, Damle NK, Ledbetter JA. Binding of the B-cell activation antigen B7 to CD28 costimulates T-cell proliferation and interleukin-2 mRNA accumulation. J Exp Med. 1991; 173:721-730. (Biology).
  17. Linsley PS, Greene JL, Brady W, Bajorath J, Ledbetter JA, Peach R. Human B7-1 (CD80) and B7-2 (CD86) bind with similar avidities but distinct kinetics to CD28 and CTLA-4 receptors. Immunity. 1994; 1:793-801. (Biology).
  18. Lum LG, Orcutt-Thordarson N, Seigneuret MC, Hansen JA. In vitro regulation of immunoglobulin synthesis by T-cell subpopulations defined by a new human T-cell antigen (9-3). Cell Immunol. 1982; 72:122-129. (Biology).
  19. Maino VC, Picker LJ. Identification of functional subsets by flow cytometry: intracellular detection of cytokine expression.. Cytometry. 1998; 34(5):207-15. (Biology). View Reference
  20. Maino VC. Rapid assessment of antigen induced cytokine expression in memory T cells by flow cytometry.. Vet Immunol Immunopathol. 1998; 63(1-2):199-207. (Biology). View Reference
  21. McMichael AJ, Gotch FM. T-cell antigens: new and previously defined clusters. In: McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987:31-62.
  22. Morishita Y, Sao H, Hansen JA, Martin PJ. A distinct subset of human CD4+ cells with a limited alloreactive T cell receptor repertoire. Immunol Today. 1989; 143:2783-2789. (Biology).
  23. Olive D, Cerdan C, Costello R, et al. CD28 and CTLA-4 cluster report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:360-370.
  24. Pitcher CJ, Quittner C, Peterson DM, et al. HIV-1-specific CD4+ T cells are detectable in most individuals with active HIV-1 infection, but decline with prolonged viral suppression.. Nat Med. 1999; 5(5):518-25. (Biology). View Reference
  25. Reiter C. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:352-353.
  26. Rotteveel FT, Kokkelink I, van Lier RA, et al. Clonal analysis of functionally distinct human CD4+ T cell subsets. J Exp Med. 1988; 168(5):1659-1673. (Biology). View Reference
  27. Suni MA, Picker LJ, Maino VC. Detection of antigen-specific T cell cytokine expression in whole blood by flow cytometry.. J Immunol Methods. 1998; 212(1):89-98. (Biology). View Reference
  28. Topp MS, Riddell SR, Akatsuka Y, Jensen MC, Blattman JN, Greenberg PD. Restoration of CD28 expression in CD28-CD8+ memory effector T cells reconstitutes antigen-induced IL-2 production. J Exp Med. 2003; 198:947-955. (Biology).
  29. Turka LA, Linsley PS, Paine R, Schieven GL, Thompson CB, Ledbetter JA. Signal transduction via CD4, CD8, and CD28 in mature and immature thymocytes: implications for thymic selection. J Immunol. 1991; 146:1428-1436. (Biology).
  30. Van Lier RAW, Brouwer M, DeJong R, Groot M, De Groot E, Aarden L. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:353-355.
  31. Waldrop SL, Davis KA, Maino VC, Picker LJ. Normal human CD4+ memory T cells display broad heterogeneity in their activation threshold for cytokine synthesis.. J Immunol. 1998; 161(10):5284-95. (Biology). View Reference
  32. Walker EB, Haley D, Miller W, et al. gp100(209-2M) peptide immunization of human lymphocyte antigen-A2+ stage I-III melanoma patients induces significant increase in antigen-specific effector and long-term memory CD8+ T cells.. Clin Cancer Res. 2004; 10(2):668-80. (Biology). View Reference
  33. Werwitzke S, Tiede A, Drescher BE, Schmidt RE, Witte T. CD8 β/CD28 expression defines functionally distinct populations of peripheral blood T lymphocytes. Clin Exp Immunol. 2003; 133:334-343. (Biology).
  34. van Lier RA, Brouwer M, Zeijlemaker WR, Aarden L. McMichael AJ, ed. Leucocyte Typing III: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1986:170-172.
  35. van Lier RAW, Brouwer M, Aarden LA. Signals involved in T cell activation, T cell proliferation induced through the synergistic action of anti-CD28 and anti-CD2 monoclonal antibodies. Eur J Immunol. 1988; 18:167-172. (Biology).
View All (35) View Less
337181 Rev. 1

 

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