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Purified Rabbit Anti- Bim
Purified Rabbit Anti- Bim

Western blot analysis of Bim. Lysates from K-562 human leukemia cells were probed with anti-Bim antibody (Cat.No. 559685) at 1 µg/ml (lane 1), 0.5 µg/ml (lane 2), and 0.25 µg/ml (lane 3). Bim was detected as a single band at ~23 kDa.

Western blot analysis of Bim. Lysates from K-562 human leukemia cells were probed with anti-Bim antibody (Cat.No. 559685) at 1 µg/ml (lane 1), 0.5 µg/ml (lane 2), and 0.25 µg/ml (lane 3). Bim was detected as a single band at ~23 kDa.

Product Details
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BD Pharmingen™
Human (QC Testing), Mouse, Rat (Reported)
Rabbit Ig
Human and mouse Bim peptide aa. 22-40
Western blot (Routinely Tested)
23 kDa
0.5 mg/ml
AB_397305
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The polyclonal antibody was purified from antiserum by negative adsorption and affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

Applications include western blot analysis (0.5-1 µg/ml). Human Jurkat T (ATCC TIB-152) or K-562 leukemia cell lines (ATCC CCL-243) are suggested as positive controls.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
559685 Rev. 4
Antibody Details
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Poly1188

The Bcl-2 family of proteins plays a pivotal role in determining the life and death of a cell. Members of the Bcl-2 family all possess at least one of four conserved Bcl-2 homology domains (BH1-BH4) which mediate protein-protein interactions. They can be divided into molecules which have either an anti-apoptotic or pro-apoptotic function. The ratio between these two groups of molecules can help determine whether a cell lives or dies. The majority of anti-apoptotic family members contain common BH1-BH4 domains and are most similar to Bcl-2. Pro-apoptotic family members can be further divided into two subfamilies (i) Bax, which also contains Bak and Bok; these proteins have similar sequence motifs in their BH1-BH3 domains, and (ii) "BH3 only domain" proteins which include Bad, Bid, Bik/Nbk, Blk, Hrk/DP5, and NIP3. A novel "BH3 only domain" protein designated Bim (BOD) was identified from human, mouse and rat by screening a cDNA expression library with a Bcl-2 protein probe. Bim induces apoptosis and binding to Bcl-XL and Bcl-w inhibits its apoptotic activity. Bim is expressed in a number of B and T cell lines, as measured by Northern blot analysis. Bim has a C-terminal hydrophobic domain which allows Bim to be associated with cytoplasmic membranes. Further studies have shown that Bim is associated with the microtubule dynein light chain motor complex via the cytoplasmic LC8 dynein light chain. Bim dissociates from the dynein motor-complex upon receiving an apoptotic signal, allowing both Bim and LC8 to translocate to the mitochondria where Bim can counteract the effect of Bcl-2-like proteins. Bim is detected at approximately 23 kDa in western blot analysis. Polyclonal rabbit anti-Bim antibody recognizes human, mouse and rat Bim. A peptide corresponding to amino acids 22 to 40 of human and mouse Bim was used as the immunogen. This sequence differs from rat Bim by only one amino acid.

559685 Rev. 4
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
559685 Rev.4
Citations & References
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Development References (6)

  1. Adams JM, Cory S. The Bcl-2 protein family: arbiters of cell survival. Science. 1998; 281(5381):1322-1326. (Biology). View Reference
  2. Gross A, McDonnell JM, Korsmeyer SJ. BCL-2 family members and the mitochondria in apoptosis. Genes Dev. 1999; 13(15):1899-1911. (Biology). View Reference
  3. Hsu SY, Lin P, Hsueh AJ. BOD (Bcl-2-related ovarian death gene) is an ovarian BH3 domain-containing proapoptotic Bcl-2 protein capable of dimerization with diverse antiapoptotic Bcl-2 members. Mol Endocrinol. 1998; 12(9):1432-1440. (Biology). View Reference
  4. O'Connor L, Strasser A, O'Reilly LA, et al. Bim: a novel member of the Bcl-2 family that promotes apoptosis. EMBO J. 1998; 17(2):384-395. (Immunogen: Western blot). View Reference
  5. Oltvai ZN, Milliman CL, Korsmeyer SJ. Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell. 1993; 74(4):609-619. (Biology). View Reference
  6. Puthalakath H, Huang DC, O'Reilly LA, King SM, Strasser A. The proapoptotic activity of the Bcl-2 family member Bim is regulated by interaction with the dynein motor complex. Mol Cell. 1999; 3(3):287-296. (Biology). View Reference
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559685 Rev. 4

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.