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Purified Mouse Anti-Zn-α2-glycoprotein
Product Details
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BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG1
Human Zn-α2-glycoprotein aa. 7-102
Western blot (Routinely Tested)
41 kDa
250 µg/ml
AB_399720
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
612354 Rev. 1
Antibody Details
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35/Zn-α2-glycoprotein

Zn-α2-glycoprotein (ZAG) is a soluble protein that was originally isolated from human plasma. ZAG is related to class I major histocompatibility complex (MHC) proteins, which are involved in peptide presentation to cytoxic T-cells during immune surveillance. Besides plasma, ZAG is found in liver, as well as bodily fluids such as sweat, saliva, cerebrospinal fluid, seminal plasm, milk, ammniotic fluid, and urine. In addition, ZAG is found in 40% of breast carcinomas, and in varous tumor cells. In plasma, ZAG's lipid binding ability may be important for lipid store homeostasis. Additional functions of ZAG may be involved in the regulation of cell proliferation. In vitro, ZAG has ribonuclease activity that is comparable to onconase and less than RNase A. ZAG may also regulate cell adhesion, since Tu-138 oral squamouse cells can attach to a ZAG substratum. This attachment inhibits cell proliferation and may involve interaction with integrin α5β1 receptors. Thus, ZAG may be a multi-functional protein involved in lipid storage, cell adhesion, and cell differentiation.

612354 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612354 Rev.1
Citations & References
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Development References (5)

  1. Kennedy MW, Heikema AP, Cooper A, Bjorkman PJ, Sanchez LM. Hydrophobic ligand binding by Zn-alpha 2-glycoprotein, a soluble fat-depleting factor related to major histocompatibility complex proteins. J Biol Chem. 2001; 276(37):35008-35013. (Biology). View Reference
  2. Lei G, Arany I, Tyring SK, Brysk H, Brysk MM. Zinc-alpha 2-glycoprotein has ribonuclease activity. Arch Biochem Biophys. 1998; 355(2):160-164. (Biology). View Reference
  3. Lei G, Brysk H, Arany I, Tyring SK, Srinivasan G, Brysk MM. Characterization of zinc-alpha(2)-glycoprotein as a cell adhesion molecule that inhibits the proliferation of an oral tumor cell line. J Cell Biochem. 1999; 75(1):160-169. (Biology). View Reference
  4. Sanchez LM, Lopez-Otin C, Bjorkman PJ. Biochemical characterization and crystalization of human Zn-alpha2-glycoprotein, a soluble class I major histocompatibility complex homolog. Proc Natl Acad Sci U S A. 1997; 94(9):4626-4630. (Biology). View Reference
  5. Ueyama H, Niwa M, Tada T, Sasaki M, Ohkubo I. Cloning and nucleotide sequence of a human Zn-alpha 2-glycoprotein cDNA and chromosomal assignment of its gene. Biochem Biophys Res Commun. 1991; 177(2):696-703. (Biology). View Reference
View All (5) View Less
612354 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.