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Purified Mouse Anti-Synaptotagmin II
Product Details
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BD Transduction Laboratories™
Rat (QC Testing), Human, Mouse (Reactivity Confirmed in Development), Dog, Chicken (Lack of Reactivity Confirmed in Development)
Mouse IgG1
Rat Synaptotagmin II aa. 413-422
Western blot (Routinely Tested)
67 kDa
250 µg/ml
AB_1645180
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
612716 Rev. 1
Antibody Details
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26/Synaptotagmin II

Synaptotagmin I belongs to a family of C2 domain containing proteins, which include Synatotagmin II to XI and Srg1. All of the members of this protein family contain a single transmembrane region and two copies of an internal repeat that is homologous to the regulatory region of Protein Kinase C (C2A and C2B domains). Synaptotagmin I-V and IX-XI are found in brain, while synaptotagmin VI-VIII are found in non-neuronal tissues. Synaptotagmin I and II have regulatory roles in synaptic vesicle docking and fusion with the plasmalemma. Both synaptotagmin I and II act as vSNAREs that form a complex with SNAP-25 and syntaxin at the plasma membrane. This complex is required for rapid, calcium-dependent synaptic vesicle exocytosis. In addition to their roles in exocytosis, synaptotagmin I and II can bind to calcium channels, calmodulin, and the clathrin adaptor protein AP-2. Synatotagmins III, V, VI, and X cannot bind IP4 like other synaptotagmins, but like most synaptotagmins these family members form heteromers with other synaptotagmins. Synaptotagmins IV, X, and Srg1 may regulate vesicular trafficking during brain development and plasticity. In neuronal and non-neuronal tissues, synaptotagmins may be important for vesicle exocytosis, endocytosis, and trafficking, as well calcium signaling.

612716 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612716 Rev.1
Citations & References
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Development References (3)

  1. Geppert M, Archer BT 3rd, Sudhof TC. Synaptotagmin II. A novel differentially distributed form of synaptotagmin. J Biol Chem. 1991; 266(21):13548-13552. (Biology). View Reference
  2. Kida Y, Sakaguchi M, Fukuda M, Mikoshiba K, Mihara K. Membrane topogenesis of a type I signal-anchor protein, mouse synaptotagmin II, on the endoplasmic reticulum. J Cell Biol. 2000; 150(4):719-730. (Biology). View Reference
  3. Osborne SL, Herreros J, Bastiaens PI, Schiavo G. Calcium-dependent oligomerization of synaptotagmins I and II. Synaptotagmins I and II are localized on the same synaptic vesicle and heterodimerize in the presence of calcium. J Biol Chem. 1999; 274(1):59-66. (Biology). View Reference
612716 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.