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Purified Mouse Anti-Synaptojanin 1
Purified Mouse Anti-Synaptojanin 1

Western blot analysis of Synaptojanin 1 on a rat cerebrum lysate.  Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-Synaptojanin 1 antibody.

Western blot analysis of Synaptojanin 1 on a rat cerebrum lysate.  Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-Synaptojanin 1 antibody.

Product Details
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BD Transduction Laboratories™
Rat (QC Testing), Mouse (Tested in Development)
Mouse IgG1
Rat Synaptojanin 1 aa. 1145-1259
Western blot (Routinely Tested)
140-145 kDa
250 µg/ml
AB_399572
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
612248 Rev. 1
Antibody Details
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26/Synaptojanin

Phosphoinositide turnover is a mechanism of intracellular signaling that involves phosphorylation of the inositol ring to produce signaling molecules that include phosphoinositol (PI)-3-P, PI-3,4-P2, PI-3,4,5-P3, PI-4-P, and PI-4,5-P2. These moleclules function in many signaling pathways to regulate events at cell membranes, such as endocytosis, exocytosis, and cell morphology. Synaptojanin 1 is a phosphoinositide phosphatase that selectively cleaves the 3-, 4-, and 5-  phosphates from PIs. Two splice variants of synaptojanin 1 include a 145 kDa form that is found in adult brain, and a 170 kDa that is widely expressed in  non- neuronal cells. The 145 kDa form contains a Sac1 domain that has phosphoinositide phosphatase activity, a second phosphatase domain that has only PI-5 phosphatase activity (PI-5), and a proline-rich SH3-binding domain. The 170 kDa form contains these domains, as well as a second SH3-binding  consensus sequence. Synaptojanin binds many proteins implicated in endocytosis,  including amphiphysins and endophilin. Thus, synaptojanin 1 may regulate endocytosis through modification of the interactions between SH3-domain  proteins and PIs.

612248 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612248 Rev.1
Citations & References
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Development References (3)

  1. Cestra G, Castagnoli L, Dente L. The SH3 domains of endophilin and amphiphysin bind to the proline-rich region of synaptojanin 1 at distinct sites that display an unconventional binding specificity. J Biol Chem. 1999; 274(45):32001-32007. (Biology). View Reference
  2. McPherson PS, Garcia EP, Slepnev VI. A presynaptic inositol-5-phosphatase. Nature. 1996; 379(6563):353-357. (Biology). View Reference
  3. Ramjaun AR, McPherson PS. Tissue-specific alternative splicing generates two synaptojanin isoforms with differential membrane binding properties. J Biol Chem. 1996; 271(40):24856-24861. (Biology). View Reference
612248 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.