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Purified Mouse Anti-Pleckstrin
Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse (Tested in Development)
Mouse IgG1
Human Pleckstrin aa. 2-16
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Not Recommended)
47 kDa
250 µg/ml
AB_397869
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610503 Rev. 1
Antibody Details
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25/Pleckstrin

Pleckstrin was identified in platelets as a major substrate for Protein Kinase C. Both ends of the molecule are the prototypes of a widely distributed module named the "pleckstrin homology" (PH) domain that consists of seven β-strands. In pleckstrin, the PH domains are separated by 150 amino acids. PH domains are commonly found in signal transduction proteins. Although pleckstrin's role is still poorly defined, PH domains are widely used by receptors for interaction with G proteins and for membrane attachment. It is thought that PH domains regulate the binding of pleckstrin to membranes and modulate phosphoinositide metabolism by its binding to G proteins.

610503 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610503 Rev.1
Citations & References
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Development References (4)

  1. Abrams CS, Wu H, Zhao W, Belmonte E, White D, Brass LF. Pleckstrin inhibits phosphoinositide hydrolysis initiated by G-protein-coupled and growth factor receptors. A role for pleckstrin's PH domains. J Biol Chem. 1995; 270(24):14485-14492. (Biology). View Reference
  2. Elzagallaai A, Rose SD, Trifaro JM. Platelet secretion induced by phorbol esters stimulation is mediated through phosphorylation of MARCKS: a MARCKS-derived peptide blocks MARCKS phosphorylation and serotonin release without affecting pleckstrin phosphorylation. Blood. 2000; 95(3):894-902. (Clone-specific: Western blot). View Reference
  3. Schebesta M, Pfeffer PL, Busslinger M. Control of pre-BCR signaling by Pax5-dependent activation of the BLNK gene. Immunity. 2002; 17(4):473-485. (Clone-specific: Western blot). View Reference
  4. Tyers M, Rachubinski RA, Stewart MI, et al. Molecular cloning and expression of the major protein kinase C substrate of platelets. Nature. 1988; 333(6172):470-473. (Biology). View Reference
View All (4) View Less
610503 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.