Skip to main content Skip to navigation
Purified Mouse Anti-Phospholipase Cγ (pY783)
Purified Mouse Anti-Phospholipase Cγ (pY783)

Western blot analysis for Phospholipase Cγ (pY783). Jurkat cells (Human T-cell leukemia; ATCC TIB-152) were either left untreated (lane 1) or treated (lane 2) with 1 mM pervanadate, a general inhibitor of protein tyrosine phosphatases, for 15 min at 37°C. The top panel was probed with a mouse anti-Phospholipase Cγ antibody (MN 610027) and the bottom panel was probed with the mouse anti-Phospholipase Cγ (pY783) antibody at a 1:1000 dilution.

Western blot analysis for Phospholipase Cγ (pY783). Jurkat cells (Human T-cell leukemia; ATCC TIB-152) were either left untreated (lane 1) or treated (lane 2) with 1 mM pervanadate, a general inhibitor of protein tyrosine phosphatases, for 15 min at 37°C. The top panel was probed with a mouse anti-Phospholipase Cγ antibody (MN 610027) and the bottom panel was probed with the mouse anti-Phospholipase Cγ (pY783) antibody at a 1:1000 dilution.

Product Details
Down Arrow Up Arrow


BD Transduction Laboratories™
PLCγ (pY783)
Human (QC Testing), Mouse, Rat (Tested in Development)
Mouse IgG1
Human Phosphorylated PLCγ1 Peptide
Western blot (Routinely Tested)
148 kDa
250 µg/ml
AB_399772
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
612464 Rev. 1
Antibody Details
Down Arrow Up Arrow
27/Phospholipase Cγ (pY783)

The Phospholipase C (PLC) isozymes hydrolyze phosphatidyl inositol  phosphate to inositol triphosphate and diacylglycerol. The former causes release of calcium from the endoplasmic reticulum, while the latter is an activator of Protein Kinase C. Within the PLC family, PLCγ is the only member that contains SH2 and SH3 domains. These domains enable it to interact with receptor tyrosine kinases and become enzymatically activated via phosphorylation. It exists as two isoforms: 1) PLCγ1, which is ubiquitously expressed, and 2) PLCγ2, found primarily in the lymphoid system. PLCγ is essential for growth factor-induced cell motility and mitogenesis. PLCγ1-null mice exhibit retarded embryonic growth and lethality in midgestation. In addition, PDGF stimulation leads to phosphorylation of PLCγ at Tyr 783, and activation of hydrolyzing activity. Overexpression of PLCγ is evident in several forms of cancer and it has been identified as a key mediator of PDGF-dependent cellular transformation. Thus, regulation of PLCγ activity by growth factors is involved in cell growth and transformation.

612464 Rev. 1
Format Details
Down Arrow Up Arrow
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612464 Rev.1
Citations & References
Down Arrow Up Arrow

Development References (3)

  1. Chen P, Murphy-Ullrich JE, Wells A. A role for gelsolin in actuating epidermal growth factor receptor-mediated cell motility. J Cell Biol. 1996; 134(3):689-698. (Biology). View Reference
  2. Obermeier A, Tinhofer I, Grunicke HH, Ullrich A. Transforming potentials of epidermal growth factor and nerve growth factor receptors inversely correlate with their phospholipase C gamma affinity and signal activation. EMBO J. 1996; 15(1):73-82. (Biology). View Reference
  3. Yu H, Fukami K, Itoh T, Takenawa T. Phosphorylation of phospholipase Cgamma1 on tyrosine residue 783 by platelet-derived growth factor regulates reorganization of the cytoskeleton. Exp Cell Res. 1998; 243(1):113-122. (Biology). View Reference
612464 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.