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Purified Mouse Anti-Neurexin I
Purified Mouse Anti-Neurexin I

Western blot analysis of Neurexin I on a P19 (murine embryonal carcinoma) lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the anti- Neurexin I antibody.

Western blot analysis of Neurexin I on a P19 (murine embryonal carcinoma) lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the anti- Neurexin I antibody.

Product Details
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BD Transduction Laboratories™
Mouse (QC Testing), Human, Rat, Dog (Tested in Development)
Mouse IgG1
Rat Neurexin Iα aa. 1063-1184
Western blot (Routinely Tested), Immunofluorescence (Not Recommended)
165 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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Antibody Details
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17/Neurexin I

Neurexins are a family of neuron-specific cell surface proteins that contain hundreds of isoforms. These isoforms are generated from two different promoters in three different genes that code for many splice-variant transcripts. In rat, neurexins include 160-200 kDa α-neurexins and 90-100 kDa β-neurexins, which include multipe isoforms identified as neurexin I, II, or III. Full-length α-neurexins contain three repeating domains that include a left arm (A) cell interaction module (LNS), a central EGF domain, and a right arm (B) LNS. β-neurexins are identical to α-neurexins in the C-terminal region, but lack the five LNS repeats and three EGF domains of α -neurexins. Neurexin Iα was first characterized as a Ca2+-dependent receptor for α-latrotoxin, the excitatory neurotoxin in black widow spider venom. The C-terminus of Neurexin Iα interacts with the synaptic vesicle protein, synaptotagmin, while the three repeated sequences in the extracellular region have homology to cell adhesion-related proteins. Thus, Neurexin Iα may be important for cell-cell interactions and synaptic protein localization during the neuronal differentiation.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

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Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
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Citations & References
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Development References (5)

  1. Fallon L, Moreau F, Croft BG, Labib N, Gu WJ, Fon EA. Parkin and CASK/LIN-2 associate via a PDZ-mediated interaction and are co-localized in lipid rafts and postsynaptic densities in brain. J Biol Chem. 2002; 277(1):486-491. (Biology: Western blot). View Reference
  2. Hlubek MD, Stuenkel EL, Krasnoperov VG, Petrenko AG, Holz RW. Calcium-independent receptor for alpha-latrotoxin and neurexin 1alpha [corrected] facilitate toxin-induced channel formation: evidence that channel formation results from tethering of toxin to membrane. Mol Pharmacol. 2000; 57(3):519-528. (Biology). View Reference
  3. Missler M, Sudhof TC. Neurexins: three genes and 1001 products. Trends Genet. 1998; 14(1):20-26. (Biology). View Reference
  4. Sugita S, Khvochtev M, Sudhof TC. Neurexins are functional alpha-latrotoxin receptors. Neuron. 1999; 22(3):489-496. (Biology). View Reference
  5. Ushkaryov YA, Petrenko AG, Geppert M, Sudhof TC. Neurexins: synaptic cell surface proteins related to the alpha-latrotoxin receptor and laminin. Science. 1992; 257(5066):50-56. (Biology). View Reference
View All (5) View Less
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Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.