Skip to main content Skip to navigation
Purified Mouse Anti-MnSOD
Product Details
Down Arrow Up Arrow


BD Transduction Laboratories™
Manganese Superoxide Dismutase
Mouse (QC Testing), Human, Rat, Dog (Tested in Development)
Mouse IgG1
Mouse MnSOD aa. 114-220
Western blot (Routinely Tested), Immunofluorescence (Not Recommended)
25 kDa
250 µg/ml
AB_399027
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
611581 Rev. 1
Antibody Details
Down Arrow Up Arrow
19/MnSOD

Superoxide dismutase (SOD), catalase, and glutathione peroxidase are necessary for preventing the intracellular accumulation of reactive oxygen species. Three forms of SOD include the cytosolic Cu/Zn2+ SOD, the extracelluar Cu/Zn[2+] SOD, and the mitochondrial manganese (Mn) superoxide dismutase (MnSOD). In the mitochondria, MnSOD catalyzes the dismutation of two superoxide radicals, producing H2O2 and oxygen. Following protein synthesis, MnSOD is post-transcriptionally modified for transport from the cytosol into the mitochondria. In rat hepatocytes, H2O2 exposure induces MnSOD expression, while in rat lung, exposure to smoke induces expression in bronchial epithelial cells. In addition, MnSOD expression is required for PC12 cell resistance to nitric oxide (NO) toxicity and for nNOS neuron resistance to NMDA-induced NO toxicity. Superoxide radical anions may induce transcriptional activation of the MnSOD gene through PKC phosphorylation and activation of a CREB-1/ATF-1-like factor. Thus, MnSOD removal of reactive oxygen species created by exposure to toxic stimuli is thought to be critical for the survival of a variety of cell types.

This antibody is routinely tested by western blot analysis.  Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

611581 Rev. 1
Format Details
Down Arrow Up Arrow
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611581 Rev.1
Citations & References
Down Arrow Up Arrow

Development References (5)

  1. Ambrosone CB, Freudenheim JL, Thompson PA. Manganese superoxide dismutase (MnSOD) genetic polymorphisms, dietary antioxidants, and risk of breast cancer. Cancer Res. 1999; 59(3):602-606. (Biology). View Reference
  2. Gilks CB, Price K, Wright JL, Churg A. Antioxidant gene expression in rat lung after exposure to cigarette smoke. Am J Pathol. 1998; 152(1):269-278. (Biology). View Reference
  3. Gonzalez-Zulueta M, Ensz LM, Mukhina G. Manganese superoxide dismutase protects nNOS neurons from NMDA and nitric oxide-mediated neurotoxicity. J Neurosci. 1998; 18(6):2040-2055. (Biology). View Reference
  4. Kim HP, Roe JH, Chock PB, Yim MB. Transcriptional activation of the human manganese superoxide dismutase gene mediated by tetradecanoylphorbol acetate. J Biol Chem. 1999; 274(52):37455-37460. (Biology). View Reference
  5. Rohrdanz E, Kahl R. Alterations of antioxidant enzyme expression in response to hydrogen peroxide. Free Radic Biol Med. 1998; 24(1):27-38. (Biology). View Reference
View All (5) View Less
611581 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.