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Purified Mouse Anti-Human TRAF3
Purified Mouse Anti-Human TRAF3

Western blot analysis of TRAF3. 293 adenovirustransformed (lanes 1 - 2), HeLa carcinoma (lanes 3 - 4), and Jurkat T (lanes 5 - 6) cell lysates were probed with anti-human TRAF3, clone B1-6 (lanes 1, 3 - 5) or a mouse IgG1 isotype (negative) control (lanes 2,4, - 6). TRAF3 is identified as an ~64 kDa band.

Western blot analysis of TRAF3. 293 adenovirustransformed (lanes 1 - 2), HeLa carcinoma (lanes 3 - 4), and Jurkat T (lanes 5 - 6) cell lysates were probed with anti-human TRAF3, clone B1-6 (lanes 1, 3 - 5) or a mouse IgG1 isotype (negative) control (lanes 2,4, - 6). TRAF3 is identified as an ~64 kDa band.

Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse IgG1, κ
Recombinant human TRAF3 protein
Western blot (Routinely Tested)
64 kDa
0.5 mg/ml
AB_396427
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

Applications include western blot analysis (1 -2 µg /ml). Jurkat T cells ( ATCC TIB-152 ), HeLa carcinoma cells (ATCC CCL-2), and 293 adenovirus- transformed kidney cell s (ATCC CRL-1573 ) are suggested as positive control s.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
556461 Rev. 4
Antibody Details
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B1-6

TRAF3 (TNF Receptor Associated Factor 3) is a member of a group of putative signal transducing molecules which interact with several members of the TNF receptor family. TRAF3 was discovered based on its interaction with CD40 and the dominant oncogene of Epstein-Barr virus, LMP-1. As such, it also known as CRAF1 and LAP1. TRAF proteins share a novel C-terminal homology region, the "TRAF" domain, as well as an N-terminal Zinc/RING finger and/or multiple Zinc finger motifs. A leucine zipper (coiled coil) region lies between the Zinc/RING finger and TRAF domains and may promote homodimerization and  heterodimerization of TRAF proteins and receptor interaction. In vitro, TRAF3 binds directly to the cytoplasmic domains of CD30, CD40, LT-βR and TNFR type II. TRAF3 has been shown to play a role in intracellular signal  pathways associated with these receptors, including induction of cell death and of the transcription factor, NF-κB. Studies on TRAF3- deficient mice have shown that TRAF3 is required for postnatal development and for normal T-cell function. TRAF3 migrates at a molecular weight of ~64 kDa in SDS/PAGE. The B1-6 antibody recognizes an ~64 kDa band corresponding to human TRAF3. A recombinant human TRAF3 protein fragment corresponding to amino acids 571-620 was used as immunogen.

556461 Rev. 4
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
556461 Rev.4
Citations & References
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Development References (3)

  1. Cheng G, Cleary AM, Ye ZS, Hong DI, Lederman S, Baltimore D. Involvement of CRAF1, a relative of TRAF, in CD40 signaling. Science. 1995; 267(5203):1494-1498. (Biology). View Reference
  2. Mosialos G, Birkenbach M, Yalamanchili R, VanArsdale T, Ware C, Kieff E. The Epstein-Barr virus transforming protein LMP1 engages signaling proteins for the tumor necrosis factor receptor family. Cell. 1995; 10(80):3-389. (Biology). View Reference
  3. Xu Y, Cheng G, Baltimore D. Targeted disruption of TRAF3 leads to postnatal lethality and defective T-dependent immune responses. Immunity. 1996; 5(5):407-415. (Biology). View Reference
556461 Rev. 4

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.