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Purified Mouse Anti-Human SGT1
Purified Mouse Anti-Human SGT1

Western blot analysis of SGT1 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-human SGT1 antibody.

Western blot analysis of SGT1 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-human SGT1 antibody.

Product Details
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BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG2b
Human SGT1 aa. 191-306
Western blot (Routinely Tested), Immunofluorescence (Not Recommended)
45 kDa
250 µg/ml
AB_399475
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
612104 Rev. 1
Antibody Details
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29/SGT1

Maintenance of cellular function requires timely and selective degradation of key regulatory proteins. For example, progression of the mammalian cell cycle is regulated by the degradation of key cell cycle proteins via the ubiquitin pathway. Ubiquitin, a soluble protein of 76 amino acids, is enzymatically attached to an ε-NH2-Lys in a target protein. Ubiquitin-conjugated proteins are recognized and degraded by the 26S proteasome. Targeting of specific cellular proteins for degradation via the ubiquitylation pathway may require the formation of protein complexes. One group of ubiquitin enzyme containing complexes are the multisubunit cullin-containing RING E3 ubiquitin ligases, that include SCF and VCB-Cul-2 complexes. These complexes contain a RING domain protein, a cullin, multiple adaptor proteins, and ubiquitin enzymes. The SCF complex includes Skp1, SGT1, Cul-1, F-box protein, and CDC34/Ubc3. This complex preferentially ubiquitylates phosphoproteins, such as β-catenin, IκBα, and G1 cyclins. The mammalian SGT1 is an adaptor protein that can interact with Skp1 and can rescue yeast SGT1 null mutations. Thus, SGT1 may be an important component of the SCF ubiquination ligase complex.

612104 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612104 Rev.1
Citations & References
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Development References (2)

  1. Kitagawa K, Skowyra D, Elledge SJ, Harper JW, Hieter P. SGT1 encodes an essential component of the yeast kinetochore assembly pathway and a novel subunit of the SCF ubiquitin ligase complex. Mol Cell. 1999; 4(1):21-33. (Biology). View Reference
  2. Read MA, Brownell JE, Gladysheva TB, et al. Nedd8 modification of cul-1 activates SCF(beta(TrCP))-dependent ubiquitination of IkappaBalpha. Mol Cell Biol. 2000; 20(7):2326-2333. (Biology). View Reference
612104 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.