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Purified Mouse Anti-Human Caspase-9
Purified Mouse Anti-Human Caspase-9

Western blot analysis for caspase-9. A K-562 cell lysate (Human bone marrow myelogenous leukemia; ATCC CCL-243) was probed with 2 µg/mL of the Mouse Anti-Human Caspase-9 antibody.  Caspase-9 may be identified at ~ 46-48 kDa.

Western blot analysis for caspase-9. A K-562 cell lysate (Human bone marrow myelogenous leukemia; ATCC CCL-243) was probed with 2 µg/mL of the Mouse Anti-Human Caspase-9 antibody.  Caspase-9 may be identified at ~ 46-48 kDa.

Product Details
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BD Pharmingen™
ICE-LAP-6, Mch6, Apaf-3
Human (QC Testing)
Mouse IgG1, κ
Human Caspase-9 aa. 364-405 Recombinant Protein
Western blot (Routinely Tested)
46-48 kDa
0.5 mg/ml
AB_396438
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
556510 Rev. 5
Antibody Details
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B40

Caspase 9 (ICE-LAP6, Mch-6, Apaf-3) is a member of a family of cysteine proteases which play a critical role in the induction of programmed cell death. Members of this family have been grouped according to sequence homology as being either ICE-like proteases (caspases 1, 4 and 5) or Ced-3- like proteases (caspases 3, 6, 7, 9 and 10a). Caspase-9 is produced as a 48 kD precursor (procaspase-9) which contains an N-terminal prodomain with high homology to caspase-2.  Procaspase-9 may be processed into subunits which heterodimerize to form the active enzyme. Activation of caspase-9 occurs in the presence of  cytochrome c (Apaf-2), following an interaction between caspase-9 and Apaf-1. Activation may also be triggered directly by the cytotoxic T-cell protease, granzyme B.  Active caspase-9 cleaves and thus activates caspase-3 and is also a relevant target of active caspase-3.  Caspase- 9 can also cleave the nuclear protein PARP. Northern blot analysis suggests that high expression of caspase-9 is found in the heart, testis and ovary.

556510 Rev. 5
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
556510 Rev.5
Citations & References
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Development References (4)

  1. Cohen GM. Caspases: the executioners of apoptosis. Biochem J. 1997; 326(1):1-16. (Biology). View Reference
  2. Duan H, Chinnaiyan AM, Hudson PL, Wing JP, He WW, Dixit VM. ICE-LAP3, a novel mammalian homologue of the Caenorhabditis elegans cell death protein Ced-3 is activated during Fas- and tumor necrosis factor-induced apoptosis. J Biol Chem. 1996; 271(3):1621-1625. (Biology: Activation). View Reference
  3. Li P, Nijhawan D, Budihardjo I, et al. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell. 1997; 91(4):479-489. (Biology: Activation). View Reference
  4. Srinivasula SM, Fernandes-Alnemri T, et al. The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32. J Biol Chem. 1996; 271(43):27099-27106. (Biology). View Reference
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556510 Rev. 5

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.