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Purified Mouse Anti-Human Caspase-4
Purified Mouse Anti-Human Caspase-4

Western blot analysis of Caspase-4 (TX). Lysates from 293 adenovirus-transformed human kidney cells were probed with anti-Caspase-4 (clone B25-1). The B25-1 antibody identifies Caspase-4 as an ~43 kDa band.

Western blot analysis of Caspase-4 (TX). Lysates from 293 adenovirus-transformed human kidney cells were probed with anti-Caspase-4 (clone B25-1). The B25-1 antibody identifies Caspase-4 as an ~43 kDa band.

Product Details
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BD Pharmingen™
TX, Ich-2
Human (QC Testing)
Mouse IgG1, κ
Recombinant Human Caspase-4
Western blot (Routinely Tested)
43 kDa
0.5 mg/ml
AB_396425
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

Applications include western blot analysis (1-2 µg/ml). Jurkat T cells (ATCC TIB- 152) and 293 adenovirus-transformed human kidney cells (ATCC CRL-1573) are suggested as positive controls.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
556459 Rev. 4
Antibody Details
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B25-1

Caspase-4 (ICErelII, TX, ICH-2) is a 43 kDa cytosolic protein with homology to the ICE/Ced-3 family of cysteine proteases.  Functional similarity with ICE proteases include the ability of Caspase-4 to induce apoptosis when overexpressed.  Caspase-4 exhibits protease activity, cleaving both itself and the p30 ICE/caspase-1 proenzyme. However, unlike ICE/caspase-1, caspase-4 displays no IL-1β processing activity. Caspase-4 mRNA is expressed in most adult human tissues, suggesting a broad tissue distribution.  

The B25-1 antibody recognizes an ~43 kDa band corresponding to human Caspase-4 (TX). A recombinant human Caspase-4 protein fragment corresponding to amino acids 334-377 was used as immunogen.

556459 Rev. 4
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
556459 Rev.4
Citations & References
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Development References (2)

  1. Faucheu C, Diu A, Chan AW. A novel human protease similar to the interleukin-1 beta converting enzyme induces apoptosis in transfected cells. EMBO J. 1995; 14(9):1914-1922. (Biology). View Reference
  2. Munday NA, Vaillancourt JP, Ali A. Molecular cloning and pro-apoptotic activity of ICErelII and ICErelIII, members of the ICE/CED-3 family of cysteine proteases. J Biol Chem. 1995; 270(26):15870-15876. (Biology). View Reference
556459 Rev. 4

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.