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Purified Mouse Anti-DsRed with Control

Purified Mouse Anti-DsRed with Control

Clone E64-1077

(RUO)
Purified Mouse Anti-DsRed with Control

Western blot analysis of DsRed. Lysate from DsRed2 was probed with anti-DsRed (clone E64-1077, Cat. No. 551814) at concentrations of 1.0 (lane 1), 0.5 (lane 2), and 0.25 µg/ml (lane 3). DsRed is identified as a band of ~31 kDa.

Western blot analysis of DsRed. Lysate from DsRed2 was probed with anti-DsRed (clone E64-1077, Cat. No. 551814) at concentrations of 1.0 (lane 1), 0.5 (lane 2), and 0.25 µg/ml (lane 3). DsRed is identified as a band of ~31 kDa.

Product Details
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BD Pharmingen™
Western blot (Routinely Tested)
RUO
AB_394264


Description

DsRed is naturally occurring fluorescent protein from a coral of the genus Discosoma. DsRed, like green fluorescent protein (GFP) can be introduced into either cultured cells or in transgenic animals to study the localization of a specific protein. It can also be used in fusion constructs with a protein of interest to examine protein-protein interactions or as a general marker. The crystal structure of DsRed has been solved and shown that DsRed monomers exhibit similar topology to GFP, but have additional chemical modifications which account for differences in the spectra, when compared to GFP. DsRed has a longer emission/absorption wavelength than GFP. DsRed migrates at 31 kDa in SDS/PAGE. The antibody recognizes three forms of DsRed; 1, 2 and pTimer. A recombinant protein representing DsRed1 was used as the immunogen.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Applications include western blot analysis (0.25-1.0 µg/ml). DsRed2 control lysate is provided as a positive control (component 51-16616N) and approximately 10 µl is recommended to visualize DsRed by western blot analysis.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
551814 Rev. 1
Components
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Description Quantity/Size Part Number
Purified Mouse Anti-DsRed 50 µg (1 ea) 51-8115GR
DsRed Control Lysate 100 µL (1 ea) 51-16616N
551814 Rev. 1
Citations & References
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Development References (1)

  1. Wall MA, Socolich M, Ranganathan R. The structural basis for red fluorescence in the tetrameric GFP homolog DsRed. Nat Struct Biol. 2000; 7(12):1133-1138. (Biology). View Reference
551814 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Non-IVD products are For Research Use Only. Not for use in diagnostic or therapeutic procedures.

 

23-22940-00