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Purified Mouse Anti-B-Raf
Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat (Tested in Development)
Mouse IgG2a
Human B-Raf aa. 285-406
Western blot (Routinely Tested)
72/95 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
612375 Rev. 3
Antibody Details
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The Raf kinase family, A-Raf, B-Raf, and C-Raf, act as downstream effectors for Ras. These ser/thr kinases have homologous kinase domains referred to as conserved regions  (CR1, CR2, and CR3). Rafs have a critical role in the response to many growth factors including: EGF, PDGF, insulin, IL-2, IL-3, CSF-1, and GM-CSF. Raf can directly interact with Ras-GTP and subsequently become activated. This leads to Raf activation of the MAP kinase pathway by phosphorylating and activating  MEK, which then phosphorylates and activates MAP kinaes (ERK). A-Raf is a proto-onocogene that is highly expressed in urogenital and kidney tissues while B-Raf is expressed at high levels in cerebrum and testes. C-Raf appears to be uniformly and ubiquitously expressed. Cell transformation by A-Raf has been shown to be independent of p21ras, suggesting the possibility  of an independent signal transduction pathway. B-Raf is expressed as multiple isoforms that range from 69-72 kDa and 79-99 kDa. In hematopoietic cells, B-Raf is activated by EPO, GM-CSF, and SCF. Thus, the Raf kinases are important Ras effectors involved in growth factor signaling in a wide variety of cells and tissues.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

612375 Rev. 3
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
612375 Rev.3
Citations & References
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Development References (4)

  1. Barnier JV, Papin C, Eychene A, Lecoq O, Calothy G. The mouse B-raf gene encodes multiple protein isoforms with tissue-specific expression. J Biol Chem. 1995; 270(40):23381-23389. (Biology). View Reference
  2. Eychene A, Dusanter-Fourt I, Barnier JV. Expression and activation of B-Raf kinase isoforms in human and murine leukemia cell lines. Oncogene. 1995; 10(6):1159-1165. (Biology). View Reference
  3. Sithanandam G, Kolch W, Duh FM, Rapp UR. Complete coding sequence of a human B-raf cDNA and detection of B-raf protein kinase with isozyme specific antibodies. Oncogene. 1990; 5(12):1775-1780. (Biology). View Reference
  4. Yuryev A, Ono M, Goff SA, Macaluso F, Wennogle LP. Isoform-specific localization of A-RAF in mitochondria. Mol Cell Biol. 2000; 20(13):4870-4878. (Biology). View Reference
View All (4) View Less
612375 Rev. 3

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.