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Purified Mouse Anti-Arc
Product Details
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BD Transduction Laboratories™
Activity-Regulated Cytoskeleton-associated protein
Rat (QC Testing), Human, Mouse (Tested in Development)
Mouse IgG1
Rat Arc aa. 264-385
Western blot (Routinely Tested)
55 kDa
250 µg/ml
AB_399886
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
612603 Rev. 3
Antibody Details
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40/Arc

Immediate early genes (IEGs) are the first to be transcribed upon cellular stimulation. Two groups of these genes have been characterized: those which encode transcription factors that indirectly influence cellular activity, and those which encode effector proteins that directly affect cellular activity. Arc (Activity-Regulated Cytoskeleton-associated protein) encodes an effector protein associated with the actin cytoskeletal matrix. In activated neurons of the hippocampus, Arc gene expression is rapidly induced. Arc protein is concentrated in the actin cortex, adjacent to the plasma membrane of the cell body and dendrites. Arc has a putative role in the maintenance of neuronal plasticity and in activity-dependent changes in dendrite function. Cocaine and D2 receptor antagonists are known to trigger the production and localization of Arc to dendrites and neuronal cell bodies.

612603 Rev. 3
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612603 Rev.3
Citations & References
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Development References (2)

  1. Lyford GL, Yamagata K, Kaufmann WE. Arc, a growth factor and activity-regulated gene, encodes a novel cytoskeleton-associated protein that is enriched in neuronal dendrites. Neuron. 1995; 14(2):433-445. (Biology). View Reference
  2. Vazdarjanova A, McNaughton BL, Barnes CA. Experience-dependent coincident expression of the effector immediate-early genes arc and Homer 1a in hippocampal and neocortical neuronal networks. J Neurosci. 2002; 22(23):10067-10071. (Biology). View Reference
612603 Rev. 3

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.