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MAP Kinase Activation Sampler Kit

MAP Kinase Activation Sampler Kit

(RUO)
MAP Kinase Activation Sampler Kit
IP: nat = native condition, den = denaturing conditions Dilutions are recommended based on western blotting on the indicated positive control. This kit includes 10 µg of each antibody listed at a concentration of 250 µg/ml. No substitutions allowed.
IP: nat = native condition, den = denaturing conditions Dilutions are recommended based on western blotting on the indicated positive control. This kit includes 10 µg of each antibody listed at a concentration of 250 µg/ml. No substitutions allowed.
Product Details
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BD Transduction Laboratories™
Western blot (Routinely Tested)
RUO
AB_2870077


Description

The family of serine/threonine kinases known as mitogen-activated protein kinases (MAP kinases) are activated after cell stimulation by a variety of hormones and growth factors, as well as during cell responses to stress. Three important kinases in this family include ERK1/2, JNK/SAPK1, and p38. These kinases all contain dual phosphorylation sites that mediate their activation. ERK1 and ERK2 (44/42 kDa) are Ser/Thr kinases activated in cells following stimulation with growth factors such as insulin, the platelet-derived growth factor, or the epidermal growth factor. In rat, these proteins are phosphorylated at Thr-202/Tyr-204 and Thr-183/Tyr-185, respectively. ERK1 and 2 have been implicated in growth factor signaling, as well as other signal transduction pathways. Growth factor stimulation leads to activation of Ras and Raf, leading to phosphorylation of MEK1 (MAPK/ERK kinase) which, in turn, activates ERK via dual phosphorylation. Thus, ERK1 and 2 are critical kinases in multiple signal transduction pathways that regulate cell growth and differentiation. External stimuli, like endotoxins, UV irradiation, heat, and hyperosmolarity, induce an array of cellular responses that culminate with gene expression, ultimately dictating an adaptation to the new environment. Stress signals activate both p38 and JNK/SAPK1 kinases. Activation of JNK/SAPK requires the phosphorylation of Thr-183/Tyr-185 by MKK4 and MKK7. Active JNK/SAPK phosphorylates other kinases and multiple transcription factors that induce expression of genes, such as proinflammatory cytokines. p38 MAP kinases include p38α, β, γ, and δ. Activation of p38 MAPK is mediated through phosphorylation of Thr-180/ Tyr-182 by MKK3, MKK4, and MKK6. This leads to the activation of multiple transcription factors (NF-κB, ATF-2, Elk-1, and CHOP) that induce expression of many different genes. Thus, these MAP kinases have important roles in many different signal transduction pathways where they regulate both cytoplasmic and nuclear proteins.

Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
612544 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.