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Jurkat Apoptotic Lysate Set I

Jurkat Apoptotic Lysate Set I

(RUO)
Jurkat Apoptotic Lysate Set I
Western blot analysis of PARP. Untreated (lanes 1-3) and camptothecin treated (lanes 4-6) Jurkat cell lysates (Human T-cell leukemia; ATCC TIB-152) were probed with a mouse anti-PARP antibody (Cat. No. 556362) at concentrations of 2.0 µg/mL (lanes 1,4), 1.0 µg/mL (lanes 2,5) and 0.5 µg/mL (lanes 3,6). Intact PARP is identified as a band of ~ 113-116 kDa, and cleaved PARP as a band of ~85-89 kDa.
Western blot analysis of PARP. Untreated (lanes 1-3) and camptothecin treated (lanes 4-6) Jurkat cell lysates (Human T-cell leukemia; ATCC TIB-152) were probed with a mouse anti-PARP antibody (Cat. No. 556362) at concentrations of 2.0 µg/mL (lanes 1,4), 1.0 µg/mL (lanes 2,5) and 0.5 µg/mL (lanes 3,6). Intact PARP is identified as a band of ~ 113-116 kDa, and cleaved PARP as a band of ~85-89 kDa.
Product Details
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BD Pharmingen™
Western blot (Routinely Tested)
RUO
AB_2868913


Description

The Jurkat Apoptotic Lysate Set I is designed to be used as a western blot positive control for detecting antigens expressed during apoptosis. Jurkat cells in log-phase growth were either left untreated or treated with 4 µM camptothecin for 4 hours to induce apoptosis. The cells were collected, lysed in lysis buffer [1% SDS, 1 mM sodium orthovanadate, 10 mM Tris (pH 8.0)], and aliquoted in SDS sample buffer.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
550959 Rev. 1
Components
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Description Quantity/Size Part Number EntrezGene ID
Camptothecin Treated Jurkat Lysate 500 µg (1 ea) 51-16606Y N/A
Jurkat Control Lysate 500 µg (1 ea) 51-16506Y N/A
550959 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.