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Biotin Mouse Anti-Cox-2
Biotin Mouse Anti-Cox-2

Western blot analysis of Cox-2 on lysate from mouse macrophages treated with IFNγ and LPS. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-Cox-2.

Western blot analysis of Cox-2 on lysate from mouse macrophages treated with IFNγ and LPS. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-Cox-2.

Product Details
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BD Transduction Laboratories™
PGHS-2, Cyclooxygenase-2
Mouse (QC Testing), Human, Chicken (Tested in Development)
Mouse IgG1
Rat Cox-2 aa. 368-604
Western blot (Routinely Tested)
70 kDa
250 µg/ml
AB_397604
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610205 Rev. 1
Antibody Details
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33/Cox-2

Cyclooxygenase (Cox) is also known as prostaglandin H synthase or PGH synthase (E.C. 1.14.99.1). It catalyzes the conversion of arachidonate to prostaglandin H2 (PGH2), the precursor of PGE2, PGF2α, PGD2, prostacyclin, and thromboxane A2. Cox actually has two different enzymatic activities: a cyclooxygenase that mediates the formation of PGG2 from oxygen and  arachidonate and a hydroperoxidase that catalyzes a reduction of PGG2 yielding PGH2. Two Cox genes, Cox-1 and Cox-2, have been isolated in several species. A 4kb mRNA encodes the 604 amino acid Cox-2 protein. The two human Cox isoenzymes are 61% identical in amino acid composition with the active sites being highly conserved. Cox-2 mRNA and protein levels are induced by serum, lipopolysaccharides, growth factors, human chorionic gonadotropin and phorbol  testers in various mammalian cell types. It has been shown that interleukin-1α (IL-1α) induces increased levels of Cox-2 mRNA and protein in human endothelial cells. The sustained increase in Cox-2 is apparently due (at least in part) to IL-1α increasing the stability of Cox-2 mRNA. This type of regulatory mechanism may play an important role in chronic inflammatory conditions.

610205 Rev. 1
Format Details
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Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
610205 Rev.1
Citations & References
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Development References (5)

  1. Hla T, Neilson K. Human cyclooxygenase-2 cDNA. Proc Natl Acad Sci U S A. 1992; 89(16):7384-7388. (Biology). View Reference
  2. Marcheselli VL, Bazan NG. Sustained induction of prostaglandin endoperoxide synthase-2 by seizures in hippocampus. Inhibition by a platelet-activating factor antagonist. J Biol Chem. 1996; 271(40):24794-24799. (Clone-specific: Western blot). View Reference
  3. Pritchard KA Jr, O'Banion MK, Miano JM. Induction of cyclooxygenase-2 in rat vascular smooth muscle cells in vitro and in vivo. J Biol Chem. 1994; 269(11):8504-8509. (Biology). View Reference
  4. Vane JR, Mitchell JA, Appleton I. Inducible isoforms of cyclooxygenase and nitric-oxide synthase in inflammation. Proc Natl Acad Sci U S A. 1994; 91(6):2046-2050. (Biology). View Reference
  5. Xie QW, Cho HJ, Calaycay J, et al. Cloning and characterization of inducible nitric oxide synthase from mouse macrophages. Science. 1992; 256(5054):225-228. (Biology). View Reference
View All (5) View Less
610205 Rev. 1

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