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Bcl-2 Related Sampler Kit

Bcl-2 Related Sampler Kit

(RUO)
Bcl-2 Related Sampler Kit

IP: nat = native condition, den = denaturing conditions

Dilutions are recommended based on western blotting on the indicated positive control.

This kit includes 10 µg of each antibody listed at a concentration of 250 µg/ml. No substitutions allowed.

IP: nat = native condition, den = denaturing conditions

Dilutions are recommended based on western blotting on the indicated positive control.

This kit includes 10 µg of each antibody listed at a concentration of 250 µg/ml. No substitutions allowed.

Product Details
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BD Transduction Laboratories™
250 µg/ml
Western blot (Routinely Tested)
RUO
AB_697894


Description

Members of the Bcl-2 protein family contains 1 to 4 bcl-2 homology domains (BH1-4), and in some cases, C-terminal hydrophobic  regions that promote membrane binding. Bcl-2 is the prototypic anti-apoptotic gene that encodes two protein products, Bcl-2a and Bcl-2b, which differ at their C-terminal hydrophobic regions that localize them to the mitochrondrial membrane. Bcl-2 inhibition of apoptosis is modulated by homodimerization or heterodimirezetion with Bax, Bad and Beclin. Like Bcl-2, Bcl-xL inhibits cell death. Bcl-x proteins include, bcl-xL and bcl-xS, which arise from alternate 5' splice sites located within the first coding exon of the bcl-x gene. Bcl-xL is 233 aa, and is similar in size and structure to Bcl-2. The bcl-xS polypeptide shows a deletion of 63 aa, which consist of the region of Bcl-xL with the highest degree of amino acid identity to Bcl-2. Bcl-xS inhibits Bcl-2 mediated cell survival. Anti-apoptotic functions of Bcl-2 and Bcl-x are regulated through interactions with Bax, Bak, Bad, Bid, Bim, and Bik. Bax contains the conserved BH1 and BH2 domains, which allow for its homodimerization or heterodimerization with Bcl-2. The ratio of Bax homodimers to Bax/Bcl-2 heterodimers is an apoptotic checkpoint. Bad contains BH1 and BH2 domains, and dimerizes with Bcl-xL and Bcl-2. Bid contains a BH3 domain, but does not contain other Bcl-2 family domains. Caspase-8 cleavage of Bid produces a 15 kDa C-terminal fragment and a 6.5 kDa N-terminal fragment. The C-terminal fragment translocates to the mitochondria and promotes the release of cytochrome C during apoptosis.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
612742 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.