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Agarose Protein A

Agarose Protein A

(RUO)
Product Details
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BD Transduction Laboratories™
Immunoprecipitation/Western blot (Routinely Tested)
AB_2870027
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Recommended Assay Procedures

Immunoprecipitation: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Agarose_Conjugates.shtml

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
610437 Rev. 1
Antibody Details
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Protein A has been reported to bind strongly to rabbit IgG and mouse IgG2 isotypes. However, protein A does not bind mouse IgM and weakly binds IgG1. When conjugated to agarose, protein A acts as a solid matrix facilitating the immunoprecipitation of antigen-antibody complexes.

Protein A was coupled to agarose at a concentration of 2 mg/ml of hydrated agarose.

This reagent is packaged as a 1 ml slurry containing 0.5 ml of settled agarose in phosphate-buffered saline with 1.5 mM sodium azide.

610437 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.