Purified Mouse Anti-Jun

The cellular Jun (c-Jun) and its viral counterpart, v-Jun, are efficient  transcriptional activators.  Both c-Jun and v-Jun are sequence-specific DNA-binding proteins that recognize the same sequence motif, termed TRE (TPA responsive element). c-Jun does not recognize this motif unless it forms a heteromeric complex with c-fos, forming the transcription factor AP1. Jun family members JunB and JunD are 44% and 45% identical to c-Jun on the protein level, respectively. Jun family members are highly conserved between species. There is 98% amino acid identity between human and mouse c-Jun, and 45% between chicken and mouse c-Jun. There is 98% amino acid homology between human and mouse JunB, 95% between human and rat JunB, and 99.5% between mouse and rat JunB. There is 77% amino acid homology between human and mouse JunD and 71% between chicken and mouse JunD.

G56-206 recognizes Jun family members including c-Jun, JunB, and JunD. A truncated recombinant protein consisting of the C-terminal half of c-Jun was used as immunogen. This region contains the c-Jun DNA binding domain. The antibody was evaluated by western blot analysis using in vitro translated c-Jun and Cos-7 monkey kidney cells transfected with recombinant c-Jun.