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Purified Mouse Anti-Human p73
Purified Mouse Anti-Human p73
Western blot analysis of p73. Lysate from 293 human embryonic kidney cells was probed with anti-p73 (clone ER-15, Cat. No. 558785). Clone ER-15 identifies p73.
Western blot analysis of p73. Lysate from 293 human embryonic kidney cells was probed with anti-p73 (clone ER-15, Cat. No. 558785). Clone ER-15 identifies p73.
Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse IgG1, κ
Human p73α:GST fusion protein aa. 380-367
Western blot (Routinely Tested), Immunoprecipitation (Reported)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

For western blot analysis use 0.5-2 µg/ml.  Detailed protocol is available at: Human cell lines including 293 adenovirus-transformed kidney cells (ATCC CRL-1573) and cos-7 SV-40 transformed monkey kidney (ATCC CRL-1651) cell lines are recommended as positive controls.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Please refer to for technical protocols.
558785 Rev. 5
Antibody Details
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p53 is a tumor suppressor which acts as an S-phase checkpoint for DNA damage. The gene for p53 is the most commonly mutated gene identified in human cancers. Recently, a new member of the p53 family, p73, has been identified. p73 is structurally homologous to p53 in several regions, including the p53 N-terminal transactivation domain and C-terminal oligomerization domains, as well as the region corresponding to the p53 DNA-binding domain. p73, when overexpressed, can promote p53-like functions, including induction of apoptosis and induction of transcription from p53-responsive promoters such as p21. Despite structural and apparent functional homology, data suggests that these proteins may have distinct functions as well. For example, viral oncoproteins such as Adenovirus E1B 55k and HPV E6, which bind to and thus inactivate p53 during the process of transformation, do not bind to p73. In addition, unlike p53, p73 expression is not induced by DNA damage, i.e., UV irradiation. Several p73 splice variants have been identified, including α (full length), β (missing exon 13), γ (missing exon 11) and δ (missing exons 11, 12, and 13). Two hybrid analysis has shown variable interaction(s) between these isoforms in vitro. Many types of normal, tumor and virally-transformed cell lines express detectable levels of p73; however, the relative expression of p73 isoforms, as well as their functional activity, appears to be differentially regulated in various cell types. p73 alpha and beta isoforms migrate at molecular weights of 80 kD (alpha), 70 kD (beta), respectively.

The ER-15 antibody reacts with human p73α and β.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

558785 Rev. 5
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
558785 Rev.5
Citations & References
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Development References (5)

  1. De Laurenzi V, Costanzo A, Barcaroli D, et al. Two new p73 splice variants, gamma and delta, with different transcriptional activity. J Exp Med. 1998; 188(9):1763-1768. (Biology). View Reference
  2. Jost CA, Marin MC, Kaelin WG Jr. p73 is a simian [correction of human] p53-related protein that can induce apoptosis. Nature. 1997; 389(6647):122-123. (Biology). View Reference
  3. Kaghad M, Bonnet H, Yang A, et al. Monoallelically expressed gene related to p53 at 1p36, a region frequently deleted in neuroblastoma and other human cancers. Cell. 1997; 90(4):809-819. (Biology). View Reference
  4. Marin MC, Jost CA, Irwin MS, DeCaprio JA, Caput D, Kaelin WG Jr. Viral oncoproteins discriminate between p53 and the p53 homolog p73. Mol Cell Biol. 1998; 18(11):6316-6324. (Clone-specific: Immunoprecipitation, Western blot). View Reference
  5. Zhu J, Jiang J, Zhou W, Chen X. The potential tumor suppressor p73 differentially regulates cellular p53 target genes. Cancer Res. 1998; 58(22):5061-5065. (Biology). View Reference
View All (5) View Less
558785 Rev. 5

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.