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Purified Mouse Anti-Human p21
Purified Mouse Anti-Human p21

Western blot analysis of p21 expression. Lysates from MCF-7 cells (50 µg/lane) were probed with anti- human p21 (clone 2G12, Cat. No. 554262) followed by alkaline phosphatase labeled anti-mouse second-step polyclonal antibodies. The antibody recognizes p21 as a 21 kDa protein.

Western blot analysis of p21 expression. Lysates from MCF-7 cells (50 µg/lane) were probed with anti- human p21 (clone 2G12, Cat. No. 554262) followed by alkaline phosphatase labeled anti-mouse second-step polyclonal antibodies. The antibody recognizes p21 as a 21 kDa protein.

Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse IgG1, κ
Human p21 fusion protein
Western blot (Routinely Tested), Immunohistochemistry-paraffin, Immunoprecipitation (Tested During Development)
21 kDa
0.5 mg/ml
AB_395331
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

Applications include immunoprecipitation. (1-2 µg/one million cells), western blot analysis (1-2 µg/ml) and immunohistochemistry of antigen unmasked, paraffin-embedded tissue sections (5-20 µg/ml). MCF-7 human breast carcinoma cells (ATCC HTB 22) are suggested as a positive control. WI-38 human lung fibroblasts (ATCC CCL 75) treated with doxorubin (Adriamycin) can also be used as a positive control.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
554262 Rev. 1
Antibody Details
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2G12

The p21 protein belongs to a class of tumor suppressors including p16 and p27 which control progression through the cell cycle by inhibiting the activity of cyclin-cdk complexes. p21 is also known as senescent cell-derived inhibitor 1 (Sdi1), wild-type p53-activated fragment 1 (Waf1), Cdk-interacting protein 1 (Cip1), p21, and p53-regulated inhibitor of Cdks (Pic1). The 2.1 kb cDNA coding for p21 was first cloned from a library made from senescent normal human foreskin fibroblasts. When introduced into proliferating foreskin fibroblasts it causes inhibition of DNA synthesis and cell cycle arrest. The p21 mRNA is expressed at higher levels in senescent fibroblasts than in actively growing cells. The resultant protein has a calculated molecular weight of 18 kDa and runs at 21 kDa in SDS/PAGE. p21 is believed to function by inhibiting the kinase activity of the cyclin-cdk complexes to which it binds. p53 has been shown to cause the induction of p21 gene, presumably by recognizing a p53 binding site identified in the promoter of the p21 gene. Clone 2G12 recognizes human p21. A full-length human p21 fusion protein was expressed in bacteria and used as immunogen. Mice were immunized and hybridomas were selected for reactivity against p21 by ELISA and western blot analysis.

554262 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
554262 Rev.1
Citations & References
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Development References (5)

  1. Hunter T. Braking the cycle. Cell. 1993; 75(5):839-841. (Biology). View Reference
  2. Noda A, Ning Y, Venable SF, Pereira-Smith OM, Smith JR. Cloning of senescent cell-derived inhibitors of DNA synthesis using an expression screen. Exp Cell Res. 1994; 211(1):90-98. (Biology). View Reference
  3. Xiong Y, Hannon GJ, Zhang H, Casso D, Kobayashi R, Beach D. p21 is a universal inhibitor of cyclin kinases. Nature. 1993; 366(6456):701-704. (Biology). View Reference
  4. el-Deiry WS, Harper JW, O'Connor PM, et al. WAF1/CIP1 is induced in p53-mediated G1 arrest and apoptosis. Cancer Res. 1994; 54(5):1169-1174. (Biology). View Reference
  5. el-Deiry WS, Tokino T, Velculescu VE, et al. WAF1, a potential mediator of p53 tumor suppression. Cell. 1993; 75(4):817-825. (Biology). View Reference
View All (5) View Less
554262 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.