Purified Mouse Anti-human Clusterin

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BD Pharmingen™ Purified Mouse Anti-human Clusterin

Name: Purified Mouse Anti-human Clusterin
Brand: BD Pharmingen™
SKU: 552886

Clone E5

(RUO)

Immunohistochemical analysis of clusterin expression in human tonsil. Formalin-fixed, paraffin-embedded sections of human tonsil were stained Purified Mouse Anti-human Clusterin (Cat. No. 552886), followed by Biotin Goat Anti-Mouse Ig (Multiple Adsorption)(Cat. No. 550337) and Streptavidin HRP (Cat. No. 550946). Dendritic cells in the follicle and basement membrane of epithelial cells and fibrocytes expressed clusterin. Magnification 20X.

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Product Details

Brand: BD Pharmingen™

Reactivity: Human (QC Testing)

Isotype: Mouse IgG1, κ

Application: Western blot (Routinely Tested), Immunohistochemistry-frozen, Immunohistochemistry-paraffin (Tested During Development)

RRID: AB_394515

Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Immunohistochemistry: Purified Mouse Anti-human Clusterin was tested for immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed and formalin-fixed, paraffin-embedded sections. The tissue tested was human tonsil. The antibody stains dendritic cells in the follicle and basement membrane of epithelial cells. The isotype control recommended for use with this antibody is Purified Mouse IgG1 κ Isotype Control (Cat. No. 550878). For optimal indirect immunohistochemical staining, the E5 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with Biotin Goat Anti-Mouse Ig (Multiple Adsorption)(Cat. No. 550337) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB Detection System (Cat. No. 550880). More conveniently, the Anti-Mouse Ig HRP Detection Kit (Cat. No. 551011), which contains Biotin Goat Anti-Mouse Ig (Multiple Adsorption), antibody diluent, Streptavidin-HRP and DAB substrate, can be used for staining. A detailed protocol of the immunohistochemical procedure is available at http://www.bdbiosciences.com/us/s/resources.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Purified Mouse IgG1 κ Isotype Control RUO

Size 1 mL Cat No. 550878

Biotin Goat Anti-Mouse Ig (Multiple Adsorption) RUO

Size 0.25 mg Cat No. 550337

Streptavidin HRP RUO

Size 50 mL Cat No. 550946

DAB Substrate Kit RUO

Size 500 Tests Cat No. 550880

Anti-Mouse Ig HRP Detection Kit RUO

Size 200 Tests Cat No. 551011

552886 Rev. 2

Antibody Details

The E5 antibody reacts with human clusterin. Clusterin was originally isolated and characterized from glomerular immune deposits of patients with renal glomerulonephritis. Clusterin, an 80 kD disulfide-linked, heterodimeric glycoprotein (previously named SP-40,40 [serum protein 40 kD,40kD]) was shown to be part of the SC5b-9 complex of complement, yet separate and distinct from the S protein. Clusterins have since been identified in a number of mammalian species and have been implicated in diverse cellular activities, however, the exact role remains to be elucidated.

552886 Rev. 2

Format Details

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

Format: Purified

552886 Rev.2

Citations & References

Development References (3)

Murphy BF, Kirszbaum L, Walker ID, d'Apice AJ. SP-40,40, a newly identified normal human serum protein found in the SC5b-9 complex of complement and in the immune deposits in glomerulonephritis. J Clin Invest. 1988; 81(6):1858-1864. (Clone-specific: Western blot). View Reference
Rosenberg ME, Silkensen J. Clusterin: physiologic and pathophysiologic considerations. Int J Biochem Cell Biol. 1995; 27(7):633-645. (Clone-specific). View Reference
Wellmann A, Thieblemont C, Pittaluga S, et al. Detection of differentially expressed genes in lymphomas using cDNA arrays: identification of clusterin as a new diagnostic marker for anaplastic large-cell lymphomas. Blood. 2000; 96(2):398-404. (Clone-specific: Western blot). View Reference

552886 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.