Purified Mouse Anti-Cytokeratin 7

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Purified Mouse Anti-Cytokeratin 7

Name: Purified Mouse Anti-Cytokeratin 7
Brand: BD Pharmingen™
SKU: 550507

Clone RCK105

(RUO)

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Scientific Resources

Western blot analysis of Cytokeratin 7. Lysate from HeLa cells was probed with anti-cytokeratin 7 (clone RCK105, Cat. No. 550507) at concentrations of 1.0 (lane 1), 0.5 (lane 2), 0.25 µg/ml (lane 3). Cytokeratin 7 is identified as a band of ~55 kDa.

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Product Details

Brand: BD Pharmingen™

Reactivity: Human (QC Testing), Mouse, Rat, Hamster, Pig, Dog (Tested in Development)

Isotype: Mouse IgG1

Immunogen: Cytokeratins from the human bladder carcinoma cell line T24

Application: Western blot (Routinely Tested), Immunohistochemistry (Reported)

Target Molecular Weight: 55 kDa

Concentration: 0.5 mg/ml

RRID: AB_2134456

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

Applications include western blot analysis. Additional applications include immunohistochemistry on methanol fixed tissue, but this application is not tested at BD Pharmingen.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

550507 Rev. 2

Antibody Details

RCK105

Intermediate filaments (IF) are a subset of cytoskeletal proteins which function to give overall structural integrity to the plasma membrane as well as organize cells into specific tissues. IF proteins can be divided into six major types based upon similarity in sequence. Type I and II IF proteins are designated keratins based upon whether they are acidic (I) or basic (II) and are characterized by their remarkable biochemical diversity. A subgroup of keratins, designated cytokeratins, are represented in epithelial tissues by at least 20 different polypeptides, designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin 7 is found in both columnar and glandular epithelium of the lung, cervix, breast, bile ducts, and in the collecting ducts in the kidney as well as mesothelium. The molecular weight of cytokeratin 7 is approximately 54 kDa in SDS/PAGE.

550507 Rev. 2

Format Details

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

Format: Purified

550507 Rev.2

Citations & References

Development References (4)

Broers JL, Ramaekers FC, Rot MK, et al. Cytokeratins in different types of human lung cancer as monitored by chain-specific monoclonal antibodies. Cancer Res. 1988; 48(11):3221-3229. (Biology). View Reference
Lodish HF. Molecular cell biology, 4th ed.. New York: W.H. Freeman; 2000:795-847.
Ramaekers F, Huysmans A, Schaart G, Moesker O, Vooijs P. Tissue distribution of keratin 7 as monitored by a monoclonal antibody. Exp Cell Res. 1987; 170(1):235-249. (Immunogen: Western blot). View Reference
Smedts F, Ramaekers F, Robben H, et al. Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia. Am J Pathol. 1990; 136(3):657-668. (Clone-specific: Immunohistochemistry). View Reference

View All (4)

550507 Rev. 2

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.