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Purified Mouse Anti-Brm
Purified Mouse Anti-Brm

Western blot analysis of Brm on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-Brm antibody.

Purified Mouse Anti-Brm

Immunofluorescence staining of a rabbit lung section.

Western blot analysis of Brm on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-Brm antibody.

Immunofluorescence staining of a rabbit lung section.

Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog, Chicken (Tested in Development)
Mouse IgG1
Human Brm aa. 1400-1586
Western blot (Routinely Tested), Immunohistochemistry, Immunoprecipitation (Tested During Development), Immunofluorescence (Not Recommended)
180 kDa
250 µg/ml
AB_397773
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
610389 Rev. 1
Antibody Details
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24/BRM

Several of the SNF and SWI genes of Saccharomyces cerevisiae encode proteins that are involved in the regulation of transcriptional activation. One of these proteins, SNF2/SWI2, has both Drosophila and mammalian homologues, designated brm. The human Brm protein has been reported to be a 180 kDa nuclear factor that acts as a transcriptional activator when fused to a heterologus DNA binding domain. Transfected Brm, expressed in cells lacking endogenous protein, can cooperate with the glucocorticoid receptor (GR) in transcriptional activation. The cooperation between Brm and GR requires the DNA binding domain of GR and the helicase domain and the P/Q-charged domain of Brm. However, Brm does not affect on several other transcription factors. The retinoblastoma protein, Rb, stimulates the transcription of a number of genes. Like Brm, Rb up-regulates glucocorticoid-receptor-mediated transcription. Brm and Rb interact in vitro and in vivo, requiring the Rb-pocket domain and the consensus Rb-binding motif of Brm.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610389 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610389 Rev.1
Citations & References
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Development References (3)

  1. Muchardt C, Yaniv M. A human homologue of Saccharomyces cerevisiae SNF2/SWI2 and Drosophila brm genes potentiates transcriptional activation by the glucocorticoid receptor. EMBO J. 1993; 12(11):4279-4290. (Biology). View Reference
  2. Singh P, Coe J, Hong W. A role for retinoblastoma protein in potentiating transcriptional activation by the glucocorticoid receptor. Nature. 1995; 374(6522):562-565. (Biology). View Reference
  3. Wong AK, Shanahan F, Chen Y, et al. BRG1, a component of the SWI-SNF complex, is mutated in multiple human tumor cell lines. Cancer Res. 2000; 60(21):6171-6177. (Biology: Western blot). View Reference
610389 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.