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Purified Mouse Anti-Ubc9
Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog, Chicken (Tested in Development)
Mouse IgG2a
Human Ubc9 aa. 26-156
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
18 kDa
250 µg/ml
AB_398072
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610748 Rev. 1
Antibody Details
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50/Ubc9

Progression of the mammalian cell cycle is primarily regulated by phosphorylation/dephosphorylation and synthesis/degradation of many key proteins. Ubiquitin, a soluble protein of 76 amino acids, is enzymatically attached to an e-NH2-Lys in a target protein. Ubiquitination is a hallmark for rapid protein degradation of the target protein in the proteosome (a cytoplasmic complex of proteases). Human homologs of the yeast ubiquitin-conjugating enzymes (Ubc) have been reported, including Ubc9. Ubc9 is 158 amino acids with an apparent molecular weight of 18kDa. Although ubiquitously expressed, the highest levels of Ubc9 are found in testis and thymus. Ubc9 was localized to the synaptonemal complex in male mouse sex chromosomes. Furthermore, Ubc9 interacts with the recombination protein Rad51, thus suggesting an important role for Ubc9 during meiosis.

610748 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610748 Rev.1
Citations & References
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Development References (4)

  1. Buschmann T, Lerner D, Lee CG, Ronai Z. The Mdm-2 amino terminus is required for Mdm2 binding and SUMO-1 conjugation by the E2 SUMO-1 conjugating enzyme Ubc9. J Biol Chem. 2001; 276(44):40389-40395. (Clone-specific: Western blot). View Reference
  2. Kovalenko OV, Plug AW, Haaf T. Mammalian ubiquitin-conjugating enzyme Ubc9 interacts with Rad51 recombination protein and localizes in synaptonemal complexes. 1996; 93(7):2958-2963. (Biology). View Reference
  3. Saitoh H, Pizzi MD, Wang J. Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358. J Biol Chem. 2002; 277(7):4755-4763. (Clone-specific: Immunofluorescence, Western blot). View Reference
  4. Wang ZY, Qiu QQ, Seufert W. Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9. J Biol Chem. 1996; 271(40):24811-24816. (Biology). View Reference
View All (4) View Less
610748 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.