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Purified Mouse Anti-Tyrosine Hydroxylase
Purified Mouse Anti-Tyrosine Hydroxylase

Western blot analysis of Tyrosine Hydroxylase on rat cerebrum lysate.  Lane 1: 1:5000, lane 2: 1:10000, lane 3: 1:20000 dilution of Tyrosine Hydroxlase.

Purified Mouse Anti-Tyrosine Hydroxylase

Immunofluorescence staining of PC12 cells.

Western blot analysis of Tyrosine Hydroxylase on rat cerebrum lysate.  Lane 1: 1:5000, lane 2: 1:10000, lane 3: 1:20000 dilution of Tyrosine Hydroxlase.

Immunofluorescence staining of PC12 cells.

Product Details
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BD Transduction Laboratories™
Rat (QC Testing), Mouse (Tested in Development)
Mouse IgG1
Rat Tyrosine Hydroxylase aa. 18-133
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
58 kDa
250 µg/ml
AB_399615
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
612300 Rev. 1
Antibody Details
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45/Tyrosine Hydroxylase

Dopamine and its metabolic products norepinephrine and epinephrine are catecholamine neurotransmitters whose function is essential for the execution of normal neural processes in the CNS and PNS. Tyrosine hydroxylase (TH) is a non-heme iron, tetrahydrobiopterin-dependent enzyme that catalzyes the conversion of tyrosine to L-dihydroxyphenylalanine (L-DOPA). This is the rate-limiting step in the biosynthesis of catecholamines. Both the development of Parkinson's disease and other neurodegenerative diseases result from loss of the ability to synthesize catecholamines. Decreases in the activity of TH have been implicated in these diseases. Nitration of TH at Tyrosine 423 has been associated with temporary loss of enzymatic activity, and TH nitration occurs in response to the Parkinsonian toxin MPTP, and following exposure to peroxynitrite. These findings implicate nitration as a potential mode of down-regulation of TH activity during neurodegenerative disease. Thus, TH is an essential enzyme for catecholamine synthesis, which is required for normal neuronal function.

612300 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612300 Rev.1
Citations & References
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Development References (2)

  1. Blanchard-Fillion B, Souza JM, Friel T. Nitration and inactivation of tyrosine hydroxylase by peroxynitrite. J Biol Chem. 2001; 276(49):46017-46023. (Biology). View Reference
  2. Salvatore MF, Waymire JC, Haycock JW. Depolarization-stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ. J Neurochem. 2001; 79(2):349-360. (Biology). View Reference
612300 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.