Skip to main content Skip to navigation
Purified Mouse Anti-TRADD
Purified Mouse Anti-TRADD

Western blot analysis of TRADD on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152).  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-TRADD antibody.

Purified Mouse Anti-TRADD

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2.2).

Western blot analysis of TRADD on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152).  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-TRADD antibody.

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2.2).

Product Details
Down Arrow Up Arrow


BD Transduction Laboratories™
TNFR1-Associated Death Domain protein
Human (QC Testing), Rat (Tested in Development)
Mouse IgG1
Human TRADD aa. 163-312
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
34 kDa
250 µg/ml
AB_397921
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Antibody Details
Down Arrow Up Arrow
37/TRADD

TNFα (Tumor Necrosis Factor) stimulates programmed cell death and NF-κB activation as a result of its binding to the TNF receptor 1 (TNFR1). Within this receptor, a sequence referred to as the "death domain" has been shown to be necessary for both of these functions. Using the yeast two-hybrid system to detect proteins which interact with the receptor through this "death domain", a 34 kDa protein was found and designated TRADD (TNFR1-Associated Death Domain protein). TRADD appears to contain no intrinsic catalytic activity.  It also contains a death domain and it has been shown to bind to FADD and RIP. Mutational analysis of TRADD demonstrates that programmed cell death and NF-κB activation are distinct and controlled independently.

Format Details
Down Arrow Up Arrow
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
Citations & References
Down Arrow Up Arrow

Development References (5)

  1. He KL, Ting AT. A20 inhibits tumor necrosis factor (TNF) alpha-induced apoptosis by disrupting recruitment of TRADD and RIP to the TNF receptor 1 complex in Jurkat T cells. Mol Cell Biol. 2002; 22(17):6034-6045. (Biology: Western blot). View Reference
  2. Hsu H, Xiong J, Goeddel DV. The TNF receptor 1-associated protein TRADD signals cell death and NF-kappa B activation. Cell. 1995; 81(4):495-504. (Biology). View Reference
  3. Morgan M, Thorburn J, Pandolfi PP, Thorburn A. Nuclear and cytoplasmic shuttling of TRADD induces apoptosis via different mechanisms. J Cell Biol. 2002; 157(156):975-984. (Biology: Immunofluorescence). View Reference
  4. Park A, Baichwal VR. Systematic mutational analysis of the death domain of the tumor necrosis factor receptor 1-associated protein TRADD. J Biol Chem. 1996; 271(16):9858-9862. (Biology). View Reference
  5. Yount GL, Afshar G, Ries S. Transcriptional activation of TRADD mediates p53-independent radiation-induced apoptosis of glioma cells. Oncogene. 2001; 20(22):2826-2835. (Biology: Western blot). View Reference
View All (5) View Less

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.