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Purified Mouse Anti-Syntaxin 6
Purified Mouse Anti-Syntaxin 6

Western blot analysis of Syntaxin 6 on a A431 cell lysate (Human epithelial carcinoma; ATCC CRL-1555).  Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-Synatixin 6 antibody.

Purified Mouse Anti-Syntaxin 6

Immunofluorescence staining of PSFK-1 cells (Human neuroectodermal tumor line; ATCC CRL-2060).

Purified Mouse Anti-Syntaxin 6

Immunohistochemical staining on a rat brain section for Syntaxin 6.  A formalin-fixed paraffin-embedded section with citrate buffer pretreatment (40X magnification).

Western blot analysis of Syntaxin 6 on a A431 cell lysate (Human epithelial carcinoma; ATCC CRL-1555).  Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-Synatixin 6 antibody.

Immunofluorescence staining of PSFK-1 cells (Human neuroectodermal tumor line; ATCC CRL-2060).

Immunohistochemical staining on a rat brain section for Syntaxin 6.  A formalin-fixed paraffin-embedded section with citrate buffer pretreatment (40X magnification).

Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog, Chicken, Frog (Tested in Development)
Mouse IgG1
Rat Syntaxin 6 aa. 6-136
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
31-35 kDa
250 µg/ml
AB_397966
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610636 Rev. 1
Antibody Details
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30/Syntaxin 6

Signal transmission between cells is often regulated by the secretion of neurotransmitters or hormones into the extracellular space. This process is  controlled by a complex pathway of membrane trafficking which leads to membrane fusion and secretion. Syntaxin 1 is involved in three important complexes that modulate the secretion process: syntaxin and n-sec-1; syntaxin, VAMP, and SNAP-25; and syntaxin, VAMP, SNAP-25, αSNAP, and NSF (20S complex). Syntaxin 6 is a 255 amino acid member of the syntaxin family. It contains a C-terminal transmembrane domain and is located at the Golgi apparatus. In addition, Syntaxin 6 displays significant (47% identity) homology with SNAP-25. In vitro, Syntaxin 6 has been reported to interact with α-SNAP. Syntaxin 6 has been reported to have a calculated molecular weight of 30.6 kDa, but has been observed to migrate in a range inbetween ~ 31-35 kDa.

610636 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610636 Rev.1
Citations & References
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Development References (4)

  1. Bock JB, Lin RC, Scheller RH. A new syntaxin family member implicated in targeting of intracellular transport vesicles. J Biol Chem. 1996; 271(30):17961-17965. (Biology). View Reference
  2. Mullock BM, Smith CW, Ihrke G, et al. Syntaxin 7 is localized to late endosome compartments, associates with Vamp 8, and Is required for late endosome-lysosome fusion. Mol Biol Cell. 2000; 11(9):3137-3153. (Biology: Blocking). View Reference
  3. Ramalho-Santos J, Moreno RD. SNAREs in mammalian sperm: possible implications for fertilization. Dev Biol. 2000; 223(1):54-69. (Biology: Electron microscopy, Immunofluorescence, Western blot). View Reference
  4. Shewan AM, van Dam EM, Martin S. SNAREs in mammalian sperm: possible implications for fertilization. Mol Biol Cell. 2003; 14(3):973-986. (Biology: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
View All (4) View Less
610636 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.