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Purified Mouse Anti-ShcC
Purified Mouse Anti-ShcC

Western blot analysis of ShcC on a rat cerebrum lysate. Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti-ShcC antibody.

Purified Mouse Anti-ShcC

Immunofluorescence staining of PFSK-1 cells (Human neuroectodermal tumor line; ATCC CRL-2060).

Western blot analysis of ShcC on a rat cerebrum lysate. Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti-ShcC antibody.

Immunofluorescence staining of PFSK-1 cells (Human neuroectodermal tumor line; ATCC CRL-2060).

Product Details
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BD Transduction Laboratories™
Rat (QC Testing), Human, Mouse (Tested in Development)
Mouse IgG1
Mouse ShcC aa. 239-374
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
69 & 55 kDa
250 µg/ml
AB_397970
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610643 Rev. 1
Antibody Details
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23/ShcC

Mammalian ShcA is expressed as three proteins of 46 kDa, 52 kDa, and 66 kDa. Each protein contains a C-terminal SH2 domain, a central glycine/proline rich sequence, and an N-terminal phosphotyrosine-binding domain (PTB). All three Shc proteins become tyrosine phosphorylated by activated growth factor receptors and oncogene tyrosine kinases. Two additional murine genes with Shc homology have been identified and named ShcB and ShcC. The ShcC gene encodes a protein of 475 amino acids and mobility of 55 kDa. Additional immunoreactive proteins have been observed with apparent molecular weights of 69 kDa and 100 kDa. These could be due to extended 5' sequences in  the ShcC gene. In contrast to widely distributed ShcA proteins, expression of ShcC is restricted to the nervous system and spleen.

610643 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610643 Rev.1
Citations & References
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Development References (3)

  1. Conti L, Sipione S, Magrassi L, et al. Shc signaling in differentiating neural progenitor cells. Nat Neurosci. 2001; 4(6):579-586. (Biology: Flow cytometry, Immunofluorescence, Western blot). View Reference
  2. O'Bryan JP, Songyang Z, Cantley L, Der CJ, Pawson T. A mammalian adaptor protein with conserved Src homology 2 and phosphotyrosine-binding domains is related to Shc and is specifically expressed in the brain. Proc Natl Acad Sci U S A. 1996; 93(7):2729-2734. (Biology). View Reference
  3. Tarr PE, Roncarati R, Pelicci G, Pelicci PG, D'Adamio L. Tyrosine phosphorylation of the beta-amyloid precursor protein cytoplasmic tail promotes interaction with Shc. J Biol Chem. 2002; 277(19):16798-16804. (Biology: Western blot). View Reference
610643 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.