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Purified Mouse Anti-Rat Nogo-A
Purified Mouse Anti-Rat Nogo-A

Western blot analysis of Nogo-A on a rat cerebellum lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti- Nogo-A antibody.

Purified Mouse Anti-Rat Nogo-A

Immunofluorescence staining of a CREF (cloned rat embryo fibroblast) lysate.

Western blot analysis of Nogo-A on a rat cerebellum lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti- Nogo-A antibody.

Immunofluorescence staining of a CREF (cloned rat embryo fibroblast) lysate.

Product Details
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BD Transduction Laboratories™
Rat (QC Testing)
Mouse IgG1
Rat Nogo-A aa. 424-627
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
220 kDa
250 µg/ml
AB_399561
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
612238 Rev. 1
Antibody Details
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17/Nogo-A

During neural development, many axons must travel long distances before reaching their dendritic targets and establishing synapses. After injury, these axonal connections can only regenerate in the peripheral nervous system, but not in the central nervous system (CNS). This difference in axon regeneration is thought to involve various inhibitory molecules found in the myelin of axons in the CNS. Nogo was identified in assays that examined fractions from myelin extracts for the antigen of monoclonal antibody IN-1, an antibody that allows modest axon regeneration after spinal cord injury. Nogo is expressed as three different proteins, Nogo-A, -B, and -C, which are members of the Reticulon family of ER anchoring proteins. Nogo-A is the full length protein, while Nogo-B contains 172 amino acids of the N-terminus and 188 amino acids of the C-terminus of Nogo-A, and Nogo-C contains only the 188 amino acid C-terminus of Nogo-A. These splice variants are all found in optic nerve, spinal cord, and cerebral cortex, but differ in expression in other neuronal and non-neuronal tissues. Thus, Nogo-A is a myelin-associated protein that may have roles in the ER, as well as during axon regeneration.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

612238 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612238 Rev.1
Citations & References
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Development References (3)

  1. Chen MS, Huber AB, van der Haar ME, et al. Nogo-A is a myelin-associated neurite outgrowth inhibitor and an antigen for monoclonal antibody IN-1. Nature. 2000; 403(6768):434-439. (Biology). View Reference
  2. GrandPre T, Nakamura F, Vartanian T, Strittmatter SM. Identification of the Nogo inhibitor of axon regeneration as a Reticulon protein. Nature. 2000; 403(6768):439-444. (Biology). View Reference
  3. Tessier-Lavigne M, Goodman CS. Perspectives: neurobiology. Regeneration in the Nogo zone. Science. 2000; 287(5454):813-814. (Biology). View Reference
612238 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.