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Purified Mouse Anti-Rat Nischarin
Purified Mouse Anti-Rat Nischarin

Western blot analysis of Nischarin.  A lysate from the rat tumor cell line PC-12 was probed with Mouse Anti-Nischarin at concentrations of 0.25, 0.12, and 0.06 µg/ml.

Western blot analysis of Nischarin.  A lysate from the rat tumor cell line PC-12 was probed with Mouse Anti-Nischarin at concentrations of 0.25, 0.12, and 0.06 µg/ml.

Product Details
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BD Pharmingen™
Rat (QC Testing), Human, Mouse (Tested in Development)
Mouse IgG1
Mouse Nischarin
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
215 kDa
0.5 mg/ml
AB_397069
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
558262 Rev. 3
Antibody Details
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50

Integrins are membrane receptors that mediate cell-cell or cell-matrix adhesion. All integrins are transmembrane heterodimers composed of α and β subunits which are connected to the cytoskeleton.  At least 20 integrins, formed from combinations of the 12 α and 9 β subunits, have been reported.  Many of these have been implicated as transducers of molecular signals.  Their roles are essential for embryonic development, tumor metastasis, cell motility, organ function, and proper immune cell function.  Nischarin is a novel protein that binds, through its 464-562 amino-acid region, to the cytoplasmic domain of integrin α5.  Expression of Nischarin is abundant in many tissues, especially brain and kidney, and its mRNA can be detected as early as day 7 in the mouse embryo.  Nischarin might act as a negative regulator of cell migration by inhibiting Rac function.  Nischarin has a predicted molecular weight of 148 kDa (NiceProt:Q9EPW8), although we detect it at 215 kDa in SDS/PAGE.  mAb 50 reacts with Nischarin in rat (routinely tested for each lot), human (tested in development only), and mouse (tested in development only) tissue.

558262 Rev. 3
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
558262 Rev.3
Citations & References
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Development References (5)

  1. Alahari SK, Lee JW, Julian RL. Nischarin, a novel protein that interacts with the integrin α5 subunit and inhibits cell migration. J Cell Biol. 2000; 151:1141-1154. (Biology).
  2. Alahari SK, Nasrallah H. A membrane proximal region of the integrin α5 subunit is important for its interaction with nischarin. Biochem J. 2004; 377:449-457. (Biology).
  3. Alahari SK, Reddig PJ, Juliano RL. The integrin-binding protein Nischarin regulates cell migration by inhibiting PAK. EMBO J. 2004; 23:2777-2788. (Biology).
  4. Alahari SK. Nischarin inhibits Rac induced migration and invasion of epithelial cells by affecting signaling cascades involving PAK. Exp Cell Res. 2003; 288:415-424. (Biology).
  5. Juliano RL, Reddig P, Alahari S, Edin M, Howe A, Aplin A. Integrin regulation of cell signalling and motility. Biochem Soc Trans. 2004; 32:443-446. (Biology).
View All (5) View Less
558262 Rev. 3

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.