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Purified Mouse Anti-Rab4
Purified Mouse Anti-Rab4

Western blot analysis of Rab4 on a rat cerebrum lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti-Rab4 antibody.

Purified Mouse Anti-Rab4

Immunofluorescence staining of L6 cells (rat skeletal muscle myoblasts; ATCC CRL-1458).

Western blot analysis of Rab4 on a rat cerebrum lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti-Rab4 antibody.

Immunofluorescence staining of L6 cells (rat skeletal muscle myoblasts; ATCC CRL-1458).

Product Details
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BD Transduction Laboratories™
Rat (QC Testing), Human, Mouse, Dog (Tested in Development)
Mouse IgG1
Human Rab4 aa. 97-213
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
25 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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Antibody Details
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The Rab proteins are small GTP-binding molecules that are localized to specific intracellular vesicles and organelles and are important for vesicular trafficking. The Rab proteins cycle between active GTP-bound and inactive GDP-bound states. Rab4 is associated with early endosomes and recycling vesicles. It is involved in the recycling of receptors to the plasma membrane and regulates membrane recycling from early endosomes. Rab4 has been reported to be membrane-bound during interphase. However, it can be found as a cytosolic protein during mitosis. It is thought that Rab4 is phosphorylated by cdc2/cyclin B during mitosis and that Ser196 on Rab4 is the recognition site of cdc2. Mutation of Ser196 results in Rab4 membrane association during mitosis in an unphosphorylated form. Together this data indicates that Rab4 is phosphorylated by cdc2 on Ser196 which releases it into the cytosol during mitosis. In addition, Rab4 may play a role in insulin-induced glucose transport into rat adipocytes. Rab4 has also been reported to be associated with vesicles that contain GLUT4, a glucose transporter isoform.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

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Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
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Citations & References
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Development References (5)

  1. Cormont M, Bortoluzzi MN, Gautier N, et al. Potential role of Rab4 in the regulation of subcellular localization of Glut4 in adipocytes. Mol Cell Biol. 1996; 16(12):6879-6886. (Biology). View Reference
  2. Huang J, Imamura T, Olefsky JM. Insulin can regulate GLUT4 internalization by signaling to Rab5 and the motor protein dynein. Proc Natl Acad Sci U S A. 2001; 98(23):13084-13089. (Biology: Functional assay). View Reference
  3. Sans N, Racca C, Petralia RS, Wang YX, McCallum J, Wenthold RJ. Synapse-associated protein 97 selectively associates with a subset of AMPA receptors early in their biosynthetic pathway. J Neurosci. 2001; 21(19):7506-7516. (Biology: Immunofluorescence). View Reference
  4. Steiner P, Sarria JC, Glauser L, Magnin S, Catsicas S, Hirling H. Modulation of receptor cycling by neuron-enriched endosomal protein of 21 kD. J Cell Biol. 2002; 157(7):1197-1209. (Biology: Immunofluorescence). View Reference
  5. Zahraoui A, Touchot N, Chardin P, Tavitian A. The human Rab genes encode a family of GTP-binding proteins related to yeast YPT1 and SEC4 products involved in secretion. J Biol Chem. 1989; 264(21):12394-12401. (Biology). View Reference
View All (5) View Less
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Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.