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Purified Mouse Anti-βPIX
Product Details
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BD Transduction Laboratories™
Rat (QC Testing), Mouse, Dog, Chicken (Tested in Development)
Mouse IgG1
Rat βPIX aa. 351-453
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
78 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611648 Rev. 1
Antibody Details
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23/bPIX

The activity of PAK family kinases is regulated through interaction with the small GTPases Cdc42 and Rac1. PAKs are activated by the GTP-bound form of Cdc-42 and Rac1, and recruitment of PAKs to focal complexes has been implicated in Cdc42- and Rac1-dependent regulation of focal contact formation. PAK-interacting exchange factor (PIX) was identified in a screen for proteins that bind PAKs. Two forms of PIX have been identified: an 85 kDa protein designated αPIX and a 78 kDa protein designated βPIX. These proteins have 80% identity in their overlapping regions, which include myosin-like, pleckstrin (PH), Dbl (DH), and SH3 domains. In addition, αPIX contains a calponin-like domain at the N-terminus. The expression of βPIX is ubiquitous, while αPIX is expressed in heart, muscle, and thymus. PIX can act as a guanine nuleotide exchange factor for Rac1 and co-transfection of βPIX, Cdc42, and αPAK results in increased αPAK activity. PIX binding to PAK is required for localization of PAKs to focal complexes and injection of βPIX leads to Rac1-dependent membrane ruffling. Thus, PIX is important for PAK localization and activity during small GTPase-dependent regulation of cell morphology.

611648 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611648 Rev.1
Citations & References
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Development References (4)

  1. Bagrodia S, Taylor SJ, Jordon KA, Van Aelst L, Cerione RA. A novel regulator of p21-activated kinases. J Biol Chem. 1998; 273(37):23633-23636. (Biology). View Reference
  2. Manser E, Loo TH, Koh CG, et al. PAK kinases are directly coupled to the PIX family of nucleotide exchange factors. Mol Cell. 1998; 1(2):183-192. (Biology). View Reference
  3. Oh WK, Yoo JC, Jo D, Song YH, Kim MG, Park D. Cloning of a SH3 domain-containing proline-rich protein, p85SPR, and its localization in focal adhesion. Biochem Biophys Res Commun. 1997; 235(3):794-798. (Biology). View Reference
  4. Turner CE, Brown MC, Perrotta JA, et al. Paxillin LD4 motif binds PAK and PIX through a novel 95-kD ankyrin repeat, ARF-GAP protein: A role in cytoskeletal remodeling. J Cell Biol. 1999; 145(4):851-863. (Biology). View Reference
View All (4) View Less
611648 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.