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Purified Mouse Anti-PI3 Kinase p110δ
Purified Mouse Anti-PI3 Kinase p110δ

Western blot analysis of PI3-Kinase p110δ expression on rat embryonic (E21) cerebrum lysate. Rat cerebrum lysate (Cat. No. 611463) was stained with Purified Mouse Anti-PI3 Kinase p110δ (Cat. No. 611014) at 1:500 (Lane 1), 1:1000 (Lane 2), and 1:2000 (Lane 3) dilutions. PI3-Kinase p110δ expression was visualized with HRP Goat Anti-Mouse Ig (Cat. No. 554002).

Purified Mouse Anti-PI3 Kinase p110δ

Immunofluorescence staining of human endothelial cells. Human endothelial cells were stained with Purified Mouse Anti-PI3 Kinase p110δ, then visualized with FITC Goat Anti-Mouse Ig (Cat. No. 554001).  

Western blot analysis of PI3-Kinase p110δ expression on rat embryonic (E21) cerebrum lysate. Rat cerebrum lysate (Cat. No. 611463) was stained with Purified Mouse Anti-PI3 Kinase p110δ (Cat. No. 611014) at 1:500 (Lane 1), 1:1000 (Lane 2), and 1:2000 (Lane 3) dilutions. PI3-Kinase p110δ expression was visualized with HRP Goat Anti-Mouse Ig (Cat. No. 554002).

Immunofluorescence staining of human endothelial cells. Human endothelial cells were stained with Purified Mouse Anti-PI3 Kinase p110δ, then visualized with FITC Goat Anti-Mouse Ig (Cat. No. 554001).  

Product Details
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BD Transduction Laboratories™
Rat (QC Testing), Human, Mouse (Tested in Development)
Mouse IgG1
Mouse PI3 Kinase p110δ aa. 73-90
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
110 kDa
250 µg/ml
AB_398327
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot: For additional technical guidance, please refer to the protocols under "Cell Biology (WB, IP, IHC, IF)" at our website:

http://www.bdbiosciences.com/us/s/resources.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
611014 Rev. 2
Antibody Details
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29/PI3 Kinase p110δ

Phosphoinositide 3 Kinase (PI3 Kinase) phosphorylates the D-3 position of the inositol ring of phosphatidylinositol, producing PtdIns(3)P, PtdIns(3,4)P2, and PtdIns (3,4,5)P3. PI3-kinase is a heterodimer of an 85 kDa regulatory subunit (p85) containing two SH2 domains and an SH3 domain, and a 110 kDa catalytic subunit (p110). Four isoforms of p110 have been identified (α , β , γ, and δ). The p110α, β isoforms are distributed ubiquitously. The p110δ isoform is found exclusively in lymphocytes and lymphoid tissues. Lymphocytes also contains p110α and both isoforms interact with p85 and are recruited to activated signaling complexes. However, several biochemical and structural differences distinguish p110δ. Unlike p110α, p110δ autophosphorylates and does not phosphorylate p85. In addition, p110δ contains a Pro-rich region, which endows it with the potential for interaction with SH3 domain-containing proteins, and a bZIP-like domain, which is a common transcription factor domain that has also been identified in certain protein kinases. With the established role of PI3-Ks in cytoskeletal rearrangements, it is thought that p110δ contributes specifically to the regulation of lymphocytes extravasation into tissues. There is a high degree of homology in the immunogen region with the P110β form (77%), and it is likely that our antibody will detect both the beta and delta forms.

611014 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611014 Rev.2
Citations & References
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Development References (1)

  1. Chantry D, Vojtek A, Kashishian A, et al. p110delta, a novel phosphatidylinositol 3-kinase catalytic subunit that associates with p85 and is expressed predominantly in leukocytes. J Biol Chem. 1997; 272(31):19236-19241. (Biology). View Reference
611014 Rev. 2

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.