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Purified Mouse Anti-Phosphoserine
Purified Mouse Anti-Phosphoserine

Immunofluorescence.

Top row:  Anti-Phosphoserine (Cat. No. 612546).  Bottom Row: Phosphoserine/threonine (Cat. No. 612548). Left panels:  untreated A431 cells control, Right panels:  A431 & CalyculinA/ Okadaic Acid (2 hour treatment).

Immunofluorescence.

Top row:  Anti-Phosphoserine (Cat. No. 612546).  Bottom Row: Phosphoserine/threonine (Cat. No. 612548). Left panels:  untreated A431 cells control, Right panels:  A431 & CalyculinA/ Okadaic Acid (2 hour treatment).

Product Details
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BD Transduction Laboratories™
Human (QC Testing), Rat (Tested in Development)
Mouse IgG1
Human Phosphoserine
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
250 µg/ml
AB_399841
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot: Typically, 1:2500 is a useful dilution for use in Western Blot. For specific procedures, please refer to our web site at http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
612547 Rev. 1
Antibody Details
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19/pSer

Protein phosphorylation of serine and threonine residues is critical for the control of protein activity involved in various cellular events. An assortment of Ser/Thr kinases and phosphatases regulate serine and threonine phosphorylation in cell signaling pathways, such as growth factor, cytokine, chemokine, and stress response. Detection of serine and threonine phosphorylation can generally be monitored by antibodies that detect phosphoserine and phosphothreonine. Our clone 19 antibody specifically recognizes phosphoserine modifications on peptides in ELISA, while our clone 22a detects both phosphoserine and phosphothreonine modifications on peptides in ELISA. These antibodies are reported to be useful for Western blot, flow cytometry, and microscopy detection of phosphoserine and phosphothreonine levels.

612547 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612547 Rev.1
Citations & References
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Development References (3)

  1. Brivanlou AH, Darnell JE Jr. Signal transduction and the control of gene expression. Science. 2002; 295(5556):813-818. (Biology). View Reference
  2. Pawson T, Scott JD. Signaling through scaffold, anchoring, and adaptor proteins. Science. 1997; 278(5346):2075-2080. (Biology). View Reference
  3. Yan JX, Packer NH, Gooley AA, Williams KL. Protein phosphorylation: technologies for the identification of phosphoamino acids. J Chromatogr A. 1998; 808(1-2):23-41. (Biology). View Reference
612547 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.