Skip to main content Skip to navigation
Purified Mouse Anti-p45/SUG1
Product Details
Down Arrow Up Arrow


BD Transduction Laboratories™
SUG1
Human (QC Testing), Mouse, Rat, Dog (Tested in Development)
Mouse IgG1
Human p45/SUG1 aa. 47-168
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
45 kDa
250 µg/ml
AB_398379
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611066 Rev. 1
Antibody Details
Down Arrow Up Arrow
35/p45

The 26S proteasome, a eukaryotic ATP-dependent protease complex, is responsible for selective degradation of malformed proteins and certain short-lived proteins. The 26S proteasome complex consists of the 20S proteasome (multifunctional protease), which is the catalytic core, and an ATPase-containing P700 regulatory complex. This complex contains five highly related putative ATPases (TBP1, TBP7, S4, MSS1, p45) that belong to the ATPase family designated AAA (ATPases Associated with a variety of cellular Activities). They share a highly conserved 200 amino acid ATPase domain (AAA module) and participate in a range of cellular functions. p45 (Trip1, hSUG1) is the homolog of the S. cerevisiae and mouse SUG1 proteins. In a ligand-enhanced manner, mouse SUG1 interacts with a range of nuclear receptors via their AF-2 domains and yeast SUG1 functions as a transcription factor that mediates the transcriptional response of the GAL4 protein. p45 is thought to be their functional homolog. Thus, in addition to its participation in the P700 regulatory complex, p45 may function as a mammalian transcriptional modulator.

611066 Rev. 1
Format Details
Down Arrow Up Arrow
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611066 Rev.1
Citations & References
Down Arrow Up Arrow

Development References (3)

  1. Akiyama K, Yokota K, Kagawa S. cDNA cloning of a new putative ATPase subunit p45 of the human 26S proteasome, a homolog of yeast transcriptional factor Sug1p. FEBS Lett. 1995; 363(1-2):151-156. (Biology). View Reference
  2. Fraser RA, Rossignol M, Heard DJ, Egly JM, Chambon P. SUG1, a putative transcriptional mediator and subunit of the PA700 proteasome regulatory complex, is a DNA helicase. J Biol Chem. 1997; 272(11):7122-7126. (Biology). View Reference
  3. Makino Y, Yogosawa S, Kanemaki M. Structures of the rat proteasomal ATPases: determination of highly conserved structural motifs and rules for their spacing. Biochem Biophys Res Commun. 1996; 220(3):1049-1054. (Biology). View Reference
611066 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.