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Purified Mouse Anti-p115
Product Details
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BD Transduction Laboratories™
Rat (QC Testing), Mouse (Tested in Development)
Mouse IgG1
Rat p115 aa. 843-955
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
115 kDa
250 µg/ml
AB_399578
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
612261 Rev. 1
Antibody Details
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15/p115

Maturation and post translational modification of proteins occurs after their biosynthesis at the endoplasmic reticulum and their transport through the Golgi apparatus. The process involves the transport of vesicles carrying the proteins through a vectorial process of vesicle budding and fusion from the cis-compartment to the medial-compartment and the trans-compartment of the Golgi apparatus. p115 is a 959 amino acid protein located at the Golgi apparatus that, with the NEM-sensitive fusion protein and the soluble NSF attachment protein (SNAP), is required for vesicle transport from the cis-compartment to the medial-compartment. p115 protein is related to the yeast Uso1p essential for the vesicular transport from the endoplasmic reticulum to the Golgi. Native p115 appears to be a homo-oligomer, with two globular heads and a tail that resemble the overall structure of myosin. p115 is extracted from the Golgi apparatus with high salt or high pH, indicative of a membrane associated protein. p115 interacts with the golgi matrix protein GM130 but this interaction is disrupted by the Golgi fragmentation during mitosis and the phosphorylation of GM130.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

612261 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612261 Rev.1
Citations & References
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Development References (3)

  1. Barroso M, Nelson DS, Sztul E. Transcytosis-associated protein (TAP)/p115 is a general fusion factor required for binding of vesicles to acceptor membranes. Proc Natl Acad Sci U S A. 1995; 92(2):527-531. (Biology). View Reference
  2. Mary S, Charrasse S, Meriane M, et al. Biogenesis of N-cadherin-dependent cell-cell contacts in living fibroblasts is a microtubule-dependent kinesin-driven mechanism. Mol Biol Cell. 2002; 13(1):285-301. (Biology: Immunofluorescence). View Reference
  3. Waters MG, Clary DO, Rothman JE. A novel 115-kD peripheral membrane protein is required for intercisternal transport in the Golgi stack. J Cell Biol. 1992; 118(5):1015-1026. (Biology). View Reference
612261 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.